Current issue
Issue 21, 2026
Online ISSN: 2466-4367
, Issue 21, (2026)
Published: 22.01.2026.
Open Access
This Special Issue of Reforesta, International Practices for Regenerating and Restoring Forest Trees by Seeding, brings together regional practice and research from North America, South America, Europe, Asia, and Oceania on using broadcast and direct seeding to create forest cover. It presents a significant range of seeding activities inclusive of species, forests, sites, and climates. Seeding has regained interest because of its scalability and cost-effectiveness, particularly for remote or inaccessible areas, sites with low productivity where planting seedling costs are prohibitive, or when aiming for a more natural forest structure. Technological innovations, particularly in seed treatments, automation, and precision seeding improve success of large-scale seed dispersal.
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Contents
28.06.2019.
Research paper
Maximizing seed germination in five species of the genus Acacia (Fabaceae Mimosaceae)
Seeds of many tree-species possess a hard seed coat which is impervious to water. These seeds often take a long time to germinate, resulting in heterogeneity and a delay in seedlings development which is an inconvenience for reforestation success. The aim of the present work was to determine the possibilities to improve the germination of five leguminous trees of the genus Acacia that have been recorded in the arid and the desert region of Algeria using sulphuric acid. A duration of 30 min of immersion in sulphuric acid improved the seed germination up to 97.5% and 99% for A. albida and A. laeta, respectively. Increasing the time of immersion (from 30 to 90 min) improved the germination percentages for A. ehrenbergiana and A. seyal seeds to 92.5% and 93.7%, respectively. Increasing this duration to 120 min had a positive effect on A. tortilis seed germination, improving the final germination rate up to 97%. Understanding of seed Germination Requirements is very important for regeneration and successful tree establishment in forest nurseries as well as for direct plantation in arid and semi-arid lands.
Abdenour Kheloufi, Faiza Zineb Boukhatem, Lahouaria Mounia Mansouri, Mohamed Djelilate
28.06.2019.
Research paper
Physio-biochemical characterization of two acacia species (A. karroo Hayn and A. saligna Labill.) under saline conditions
Drought and salinity act simultaneously in tolerance and acclimatization under saline conditions. Therefore, plants subjected to these types of stress should have developed specific structural adaptations at the early stages of development. The solution to these environmental problems is to look for species that are relatively water-efficient and resistant to recurrent episodes of various abiotic stresses such as salt stress. In this study, the salinity tolerance index, ionic homeostasis and osmoprotection were evaluated in A. karroo and A. saligna plants of 90 days old and cultured at various concentrations of NaCl for 21 days. Results showed that salt caused remarkable changes in some growth-related parameters (dry biomass) represented by the salinity tolerance index (STI). Na+, Ca2+, and RatioNa+/K+ content in the leaves increased with salinity levels, while K+ contents were significantly reduced compared to the control in both acacia species. Levels of proline, total free amino acids and reducing sugars have been accumulated considerably in the leaves. A. karroo was more salt-tolerant than A. saligna. Our results showed that the adaptability of a species to salinity is closely related to ion selectivity and biomass production. The seedlings also accumulated significantly a set of important osmolytes in leaves under salt stress, showing a marked increase in secondary metabolite accumulation. This adaptation proved very specific to each species for better survival in saline environments.
Abdenour Kheloufi, Lahouaria Mounia Mansouri, Anas Mami, Mohamed Djelilate
28.06.2019.
Research paper
TREND-RUN model application of surface temperature and its implications for South African forestry and reforestation using local weather services data
Temperature can directly and indirectly impact the livelihood of inhabitants of a country and the natural environment as a whole. The surface temperature trend approximations for South Africa (SA) were calculated using a linear-regression fitting model. The model was adapted at The University of Reunion Island and was referred to as the Trend-Run model. The geophysical signal of the model was split into a sum of oscillations, which was used to clarify most of its variability. The trend values were calculated from the residual terms as a linear function. The model used atmospheric oscillations, which included Annual (AO), Semi-Annual (SAO), Quasi-Biennial Oscillations (QBO), El Niño-Southern Oscillation (ENSO), the 11-years solar cycle-Sun Spot Number (SSN) and Indian Ocean Dipole (IOD). The South African Weather Service (SAWS) data were used for the study. Data sets over a 31-year period, from March 1980 to December 2011, were used to measure the validity of the Trend-Run model, to determine the contribution and effect of this particular oscillation, and the validity of the model. The Trend-Run model showed very high applicability to the surface temperatures in all provinces across the SA region under investigation. High coefficient of determination values between (0.70-0.91) were recorded for surface temperatures across all provinces in the country with minor variations. The AO, ENSO and SAO were the highest contributing forcings in the model, thereby showing their high relevance to the success of this model in the study area. The temperature increases are expected to negatively impact on the biomes of SA, including the forest biome. Selected tree species of Acacia, Eucalyptus and Pinus could be impacted negatively with rising temperatures, which would negatively impact on the forestry industry in SA. As expected, the model did obtain a high success rate that ranged from 70% to 91% in the areas under study, however, there was still room for improvement by the possible inclusion of additional atmospheric forcings to the model that maybe be applicable to the weather and forestry distribution in SA.
Raven Jimmy, Pramanathan Govender, Hassan Bencherif, Matthew Moodley
31.12.2019.
Research paper
Application of a PhotoThermal model for container-grown conifer seedling production
This study applied a total energy approach to model seedling growth for container-grown loblolly pine (Pinus taeda L.). Seedlings were grown in three container stocktypes representing a range of cavity volume and density patterns. These seedlings were grown under both controlled greenhouse and outside compound environmental conditions under well-defined cultural conditions. Models for temperature and light ranges were created from work on the ecophysiological performance and morphological development of loblolly pine to these atmospheric conditions. A PhotoThermal data set was created by generating hourly averages of these two environmental variables during the growing season. Light and temperature data were integrated, each weighted equally, into PhotoThermal hours (PTH) to assess the crop growth response. Loblolly pine seedling growth in both the greenhouse and outside compound was directly related to PTH. Seedling growth was also related to the container type with the largest cavity volume and lowest cavity density having the greatest growth per PTH. Application of the PhotoThermal model is discussed for growing seedlings in an operational program having multiple production steps, delivery dates and nursery locations.
Steven Grossnickle
28.12.2018.
Research paper
The effect of seed size grading on seed use efficiency and height of one-year-old container-grown Scots pine (Pinus sylvestris L.) seedlings
The seed of Pinus sylvestris L. originating from natural forest in Voronezh region, Russian Federation, was graded in four classes based on seed diameter size, using the operational equipment of screens with round openings. The ungraded seedlot was used as control. Seedlings were produced in Voronezh containerized forest nursery, from March to September 2015, following the standard procedure for Pinus sylvestris. Seed from each size class, plus control, is sown in containers with 120 cm3 cells, filled with peat-substrate, grown in greenhouse for eight weeks, followed by hardening in growing area until the end of the growing season. The results shows a week positive effect of seed grading on height of one-year old container-grown seedlings, but significant improvement of seed use efficiency by reducing the number of empty cells. Based on results of this study, we can recommend grading of Pinus sylvestris seed on two size classes.
Arthur I. Novikov, Vladan Ivetić
28.12.2018.
Review paper
Seedling Establishment on a Forest Restoration Site
Seedling field performance is affected by both their quality and reforestation site conditions. Seedlings enter the establishment phase when they start to develop root systems into the surrounding soil and are coupled to the restoration site. Once seedlings are established, their inherent growth potential is related to morphological and physiological attributes and their ecophysiological response to site environmental conditions, which ultimately determines field performance. This establishment phase is a time when seedlings developed with certain nursery cultural practices begin to respond to site conditions. This phase is also a period when silvicultural practices have created microsites intended to benefit established seedlings field performance. Seedlings can be exposed to a wide range of environmental conditions during the establishment phase, some of which may be extreme enough to exceed their ability to physiologically tolerate environmental stress. When this occurs, seedling growth on the restoration site is reduced. On the other hand, this phase can provide planted seedlings with ideal environmental conditions that allow for an optimum physiological response and maximization of their growth potential. An understanding of the ecophysiological capability of planted seedlings can ensure they have the best chance at rapid stand establishment.
Steven Grossnickle
28.12.2018.
Research paper
Alley cropping with strawberries: Two case-studies in Romania
The paper focuses on the use of alley cropping, an arable agroforestry system comprising rows of trees planted with a companion crop grown in the alleyways between the rows, in two sub-compartments (81B%: 0.75 ha, elevation 715 m asl; 81E: 3.4 ha, elevation 735 m asl) artificially regenerated at 1.80 m x 1.60 m spacing in the south-east of Transylvania (Romania) in 1997 (81B%) and 2003 (81E). The climate (Dfbx, specific to low and middle mountains) and soil (luvisol, of moderate fertility for pure or mixed Quercus petraea stands) are only moderately favourable to strawberry culture. The rows of tree species consists of Quercus rubra, Acer pseudoplatanus, Prunus avium (81B%), and Quercus petraea, Fagus sylvatica, Acer pseudoplatanus, and Larix decidua (81E). Strawberries (variety Elsanta,) were planted at 60 x 60 cm spacing in two pure rows in-between the tree rows as well as within the rows of planted trees. Both plantations were fenced, with manual topsoil hoeing up to four times a year, lighly fertilized (N, P, K complex) and chemically protected against browsing and pests. Strawberry rotation (six years, of which five years - two to six - with fruit production) was longer than the one (maximum three years) recommended in pure crops. Strawberry production reached the maximum level in years 3 or 4.
Under the local conditions, the alley cropping with strawberries had demonstrated to be a viable alternative to the 'classical' monocropping system in terms of (a) survival rate of plants (over 95 per cent), (b) initial height growth of plants (very quick, resulting from reduced competition with grasses and naturally regenerated forest trees such as pioneer species), (c) establishment of a new forest culture, the full forest cover being achieved immediately after the end of strawberry production, as well as (d) economics (early and relevant revenue from the crop).
Valeriu Norocel Nicolescu, Cornelia Hernea, Vilhelm Sandi
28.12.2018.
Research paper
Nurse plants and the regeneration niche of tree seedlings in wood-pastures from Western and North-Western Romania
Facilitation is a positive interaction demonstrated to be one of the important factors shaping the regeneration niche of trees, mostly under stressful conditions which is currently studied in the frame of complex ecological networks. The protection provided by benefactor plants for tree seedlings playing the role of beneficiaries is documented mainly in arid and semi-arid habitats or in situations where herbivores’ pressure constitutes the main stressful factor for tree regeneration. One of the iconic Transylvanian landscapes is the wood-pasture, also one of the oldest agro-forestry systems to which recent forest expansion in abandoned agricultural fields or pastures is added. The proposed work represents a preliminary investigation on the association between benefactor plants, mostly spiny shrubs (Rosa canina, Crataegus monogyna, Prunus spinosa as the most frequently encountered benefactors) and tree seedlings (Quercus spp., Tilia spp., Carpinus betulus, Fraxinus angustifolia, Pyrus pyraster as most frequently encountered beneficiaries), in four different locations from North-Western and Western Romania, wood-pastures, abandoned pastures and abandoned agricultural fields under the consideration that the main stressful factor is represented by livestock grazing. Bipartite, qualitative merged network was generated depicting the interaction between beneficiaries and benefactors. Commonly used metrics were calculated: connectivity, nestedness, modularity, betweenness centrality and centralization compared to similar facilitation networks presented in the literature. Facilitation network is characterized by high nestedness (N=0.896), lack of modularity, relatively high connectance (C=0.233), features encountered in mutualistic networks also. Betweenness centrality scores highlighted the keystone benefactor and beneficiary species, while betweenness centralization score (0.192) indicates the fact that there are several species sharing the dominant position in terms of interactions. The analysis of measurement data (seedlings’ and benefactor plants’ heights, distance from focal seedlings to nearest benefactor species and orientation) showed that there is common pattern in orientation (most of the benefactor species oriented toward South or South West) also in dimensional variability (MANOVA results).
Ecaterina Fodor, Ovidiu Haruta, Sorin Dorog
10.07.2018.
Review paper
Fertilizer trials for bareroot nurseries in North America
In North America, most tree nursery nutrition publications during the past two decades involved either container-grown stock or stock grown in greenhouses. In contrast, most bareroot nursery fertility trials in North America were published during the last century. As a result, some bareroot fertilization recommendations have remained the same since 1980 and some practices continue to be based on myths and assumptions. The bareroot nursery industry in the USA might benefit if the next generation of graduate students will consider testing old and new theories about nursery fertilization. Hopefully, they will discover new facts so that future fertilization regimes will be based on science. This paper provides various fertilizer trials that should be established in bareroot nurseries.
David B. South
10.07.2018.
Research paper
Breaking seed coat dormancy of six tree species
Breaking physical dormancy in some forest seeds is a challenge for scientists and forest managers to obtain an homogeneous germination for larger seed samples. The role played by the seed coat in seed dormancy of six trees with great interest in agroforestry (Robinia pseudoacacia, Leucaena leucocephala, Erythrostemon gilliesii, Styphnolobium japonicum, Acacia dealbata and Brachychiton populneus) was tested by the effects of the pretreatment and its duration on the performance of seed germination, by considering the final germination percentage (FGP) and the mean germination time (MGT). These parameters are estimated at various times of incubation (5, 10 and 15 days) in Petri dishes and stored in darkness at (25 ± 2°C). The pretreatment consists of an immersion of seeds in concentrated sulphuric acid during 30, 60 and 90 minutes. Sowing without pretreatment (control) revealed no germination induction for B. populneus and A. dealbata, except for R. pseudoacacia, L. leucocephala and S. japonica where the germination does not exceed 12% for all the experience duration. Generally, pretreatments were very useful to improve seed germination. The time of immersion into sulphuric acid significantly affected (P < 0.0001) the FGP and the MGT in all studied species. A duration of 30 minutes of soaking was adequate to give a very high rate of germination for L. leucocephala, E. gilliesii, S. japonica and A. dealbata with respective FGP of 100%, 95%, 100% and 100%. However, an extended duration of pretreatment of 60 minutes was necessary for a maximal germination for R. pseudoacacia and B. populneus with FGP of 85% and 100%, respectively. A prolonged duration of 90 minutes of presowing was very fatal for L. leucocephala, A. dealbata and B. populneus. An excellent germinative strength is characterized by a higher FGP and a reduced MGT.
Abdenour Kheloufi, Lahouaria Mansouri, Nada Aziz, Meriem Sahnoune, Sarra Boukemiche, Boutheina Ababsa