Current issue
Issue 21, 2026
Online ISSN: 2466-4367
, Issue 21, (2026)
Published: 22.01.2026.
Open Access
This Special Issue of Reforesta, International Practices for Regenerating and Restoring Forest Trees by Seeding, brings together regional practice and research from North America, South America, Europe, Asia, and Oceania on using broadcast and direct seeding to create forest cover. It presents a significant range of seeding activities inclusive of species, forests, sites, and climates. Seeding has regained interest because of its scalability and cost-effectiveness, particularly for remote or inaccessible areas, sites with low productivity where planting seedling costs are prohibitive, or when aiming for a more natural forest structure. Technological innovations, particularly in seed treatments, automation, and precision seeding improve success of large-scale seed dispersal.
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Contents
28.06.2019.
Research paper
Physio-biochemical characterization of two acacia species (A. karroo Hayn and A. saligna Labill.) under saline conditions
Drought and salinity act simultaneously in tolerance and acclimatization under saline conditions. Therefore, plants subjected to these types of stress should have developed specific structural adaptations at the early stages of development. The solution to these environmental problems is to look for species that are relatively water-efficient and resistant to recurrent episodes of various abiotic stresses such as salt stress. In this study, the salinity tolerance index, ionic homeostasis and osmoprotection were evaluated in A. karroo and A. saligna plants of 90 days old and cultured at various concentrations of NaCl for 21 days. Results showed that salt caused remarkable changes in some growth-related parameters (dry biomass) represented by the salinity tolerance index (STI). Na+, Ca2+, and RatioNa+/K+ content in the leaves increased with salinity levels, while K+ contents were significantly reduced compared to the control in both acacia species. Levels of proline, total free amino acids and reducing sugars have been accumulated considerably in the leaves. A. karroo was more salt-tolerant than A. saligna. Our results showed that the adaptability of a species to salinity is closely related to ion selectivity and biomass production. The seedlings also accumulated significantly a set of important osmolytes in leaves under salt stress, showing a marked increase in secondary metabolite accumulation. This adaptation proved very specific to each species for better survival in saline environments.
Abdenour Kheloufi, Lahouaria Mounia Mansouri, Anas Mami, Mohamed Djelilate
28.06.2019.
Research paper
TREND-RUN model application of surface temperature and its implications for South African forestry and reforestation using local weather services data
Temperature can directly and indirectly impact the livelihood of inhabitants of a country and the natural environment as a whole. The surface temperature trend approximations for South Africa (SA) were calculated using a linear-regression fitting model. The model was adapted at The University of Reunion Island and was referred to as the Trend-Run model. The geophysical signal of the model was split into a sum of oscillations, which was used to clarify most of its variability. The trend values were calculated from the residual terms as a linear function. The model used atmospheric oscillations, which included Annual (AO), Semi-Annual (SAO), Quasi-Biennial Oscillations (QBO), El Niño-Southern Oscillation (ENSO), the 11-years solar cycle-Sun Spot Number (SSN) and Indian Ocean Dipole (IOD). The South African Weather Service (SAWS) data were used for the study. Data sets over a 31-year period, from March 1980 to December 2011, were used to measure the validity of the Trend-Run model, to determine the contribution and effect of this particular oscillation, and the validity of the model. The Trend-Run model showed very high applicability to the surface temperatures in all provinces across the SA region under investigation. High coefficient of determination values between (0.70-0.91) were recorded for surface temperatures across all provinces in the country with minor variations. The AO, ENSO and SAO were the highest contributing forcings in the model, thereby showing their high relevance to the success of this model in the study area. The temperature increases are expected to negatively impact on the biomes of SA, including the forest biome. Selected tree species of Acacia, Eucalyptus and Pinus could be impacted negatively with rising temperatures, which would negatively impact on the forestry industry in SA. As expected, the model did obtain a high success rate that ranged from 70% to 91% in the areas under study, however, there was still room for improvement by the possible inclusion of additional atmospheric forcings to the model that maybe be applicable to the weather and forestry distribution in SA.
Raven Jimmy, Pramanathan Govender, Hassan Bencherif, Matthew Moodley
28.06.2019.
Review paper
Is the recommended pH for growing hardwood seedlings wrong?
Two schools of thought address the optimum soil pH (measured in water) for growing hardwood seedlings in bareroot nurseries. One school uses nutrient surveys in non-fertilized forests to determine the best pH range for growing seedlings in fertilized nurseries. Some students of this school believe hardwood seedlings grow best at pH 6.0 to 7.5. In contrast, another school relies on research from pH trials to conclude that fertilized hardwoods can grow well in soils that range from pH 4.5 to 6.0. This article compiles some of the findings from seedbed and greenhouse trials and attempts to use data to dispel a few myths about the “optimum pH” for growing hardwood seedlings. Greenhouse trials suggest many fertilized hardwoods grow better in acid soils (pH 4-6) than in nearly neutral soils (pH 6.0-7.5). The optimal pH for growth differs among species and, therefore, it is a myth that all hardwood seedlings grow best at pH 6 to 7.5. Most nursery managers in the southern United States grow bareroot hardwoods between pH 4.8 and 6.0.
David B. South
28.06.2019.
Research paper
Growth characteristics of one-year-old seedlings of three autochthonous oak species in suboptimal growing conditions
The paper presents the growth characteristics of one-year-old seedlings of three autochthonous oak species (Fagaceae family): Turkey oak (Quercus cerris L.), Hungarian oak (Quercus frainetto Ten.) and downy oak (Quercus pubescens Willd.) in suboptimal growing conditions. The analyzed oak species are widely distributed in Serbia. One of the most important benefits of oak species is wood production (Hungarian and Turkey oak), and they have a promising role for the use in the future regarding the climate change as all species can be encountered on dry sites. Acorns were collected in the autumn of 2017 in natural stands and a sample of 400 acorns per each oak species was immediately sown in a nursery seedbed in the form of a random block system with four replicates. At the end of 2018, the available sample of seedlings was analyzed morphometrically. The seedlings were classified according to the number of shoot growth flushes into one-flush-growth and multi-flush growth seedlings. Turkey Oak had the highest number of seedlings which also recorded higher values in total height, root collar diameter and number of leaves. These results were obtained on alkaline soil with the presence of weeds and rodents and the absence of irrigating. The presence of multi-flush growth was recorded in all three oak species. Differences between analyzed three oak species exist in these suboptimal conditions. However, in full light conditions, a similar growth pattern was recorded, indicating to the similar adaptability of the species.
Nikola Šušić, Martin Bobinac, Mirjana Šijačić-Nikolić, Andrijana Bauer-Živković, Jelena Urošević, Ivona Kerkez Janković
28.12.2018.
Research paper
The effect of seed size grading on seed use efficiency and height of one-year-old container-grown Scots pine (Pinus sylvestris L.) seedlings
The seed of Pinus sylvestris L. originating from natural forest in Voronezh region, Russian Federation, was graded in four classes based on seed diameter size, using the operational equipment of screens with round openings. The ungraded seedlot was used as control. Seedlings were produced in Voronezh containerized forest nursery, from March to September 2015, following the standard procedure for Pinus sylvestris. Seed from each size class, plus control, is sown in containers with 120 cm3 cells, filled with peat-substrate, grown in greenhouse for eight weeks, followed by hardening in growing area until the end of the growing season. The results shows a week positive effect of seed grading on height of one-year old container-grown seedlings, but significant improvement of seed use efficiency by reducing the number of empty cells. Based on results of this study, we can recommend grading of Pinus sylvestris seed on two size classes.
Arthur I. Novikov, Vladan Ivetić
28.12.2018.
Review paper
Seedling Establishment on a Forest Restoration Site
Seedling field performance is affected by both their quality and reforestation site conditions. Seedlings enter the establishment phase when they start to develop root systems into the surrounding soil and are coupled to the restoration site. Once seedlings are established, their inherent growth potential is related to morphological and physiological attributes and their ecophysiological response to site environmental conditions, which ultimately determines field performance. This establishment phase is a time when seedlings developed with certain nursery cultural practices begin to respond to site conditions. This phase is also a period when silvicultural practices have created microsites intended to benefit established seedlings field performance. Seedlings can be exposed to a wide range of environmental conditions during the establishment phase, some of which may be extreme enough to exceed their ability to physiologically tolerate environmental stress. When this occurs, seedling growth on the restoration site is reduced. On the other hand, this phase can provide planted seedlings with ideal environmental conditions that allow for an optimum physiological response and maximization of their growth potential. An understanding of the ecophysiological capability of planted seedlings can ensure they have the best chance at rapid stand establishment.
Steven Grossnickle
28.12.2018.
Research paper
Alley cropping with strawberries: Two case-studies in Romania
The paper focuses on the use of alley cropping, an arable agroforestry system comprising rows of trees planted with a companion crop grown in the alleyways between the rows, in two sub-compartments (81B%: 0.75 ha, elevation 715 m asl; 81E: 3.4 ha, elevation 735 m asl) artificially regenerated at 1.80 m x 1.60 m spacing in the south-east of Transylvania (Romania) in 1997 (81B%) and 2003 (81E). The climate (Dfbx, specific to low and middle mountains) and soil (luvisol, of moderate fertility for pure or mixed Quercus petraea stands) are only moderately favourable to strawberry culture. The rows of tree species consists of Quercus rubra, Acer pseudoplatanus, Prunus avium (81B%), and Quercus petraea, Fagus sylvatica, Acer pseudoplatanus, and Larix decidua (81E). Strawberries (variety Elsanta,) were planted at 60 x 60 cm spacing in two pure rows in-between the tree rows as well as within the rows of planted trees. Both plantations were fenced, with manual topsoil hoeing up to four times a year, lighly fertilized (N, P, K complex) and chemically protected against browsing and pests. Strawberry rotation (six years, of which five years - two to six - with fruit production) was longer than the one (maximum three years) recommended in pure crops. Strawberry production reached the maximum level in years 3 or 4.
Under the local conditions, the alley cropping with strawberries had demonstrated to be a viable alternative to the 'classical' monocropping system in terms of (a) survival rate of plants (over 95 per cent), (b) initial height growth of plants (very quick, resulting from reduced competition with grasses and naturally regenerated forest trees such as pioneer species), (c) establishment of a new forest culture, the full forest cover being achieved immediately after the end of strawberry production, as well as (d) economics (early and relevant revenue from the crop).
Valeriu Norocel Nicolescu, Cornelia Hernea, Vilhelm Sandi
28.12.2018.
Research paper
Nurse plants and the regeneration niche of tree seedlings in wood-pastures from Western and North-Western Romania
Facilitation is a positive interaction demonstrated to be one of the important factors shaping the regeneration niche of trees, mostly under stressful conditions which is currently studied in the frame of complex ecological networks. The protection provided by benefactor plants for tree seedlings playing the role of beneficiaries is documented mainly in arid and semi-arid habitats or in situations where herbivores’ pressure constitutes the main stressful factor for tree regeneration. One of the iconic Transylvanian landscapes is the wood-pasture, also one of the oldest agro-forestry systems to which recent forest expansion in abandoned agricultural fields or pastures is added. The proposed work represents a preliminary investigation on the association between benefactor plants, mostly spiny shrubs (Rosa canina, Crataegus monogyna, Prunus spinosa as the most frequently encountered benefactors) and tree seedlings (Quercus spp., Tilia spp., Carpinus betulus, Fraxinus angustifolia, Pyrus pyraster as most frequently encountered beneficiaries), in four different locations from North-Western and Western Romania, wood-pastures, abandoned pastures and abandoned agricultural fields under the consideration that the main stressful factor is represented by livestock grazing. Bipartite, qualitative merged network was generated depicting the interaction between beneficiaries and benefactors. Commonly used metrics were calculated: connectivity, nestedness, modularity, betweenness centrality and centralization compared to similar facilitation networks presented in the literature. Facilitation network is characterized by high nestedness (N=0.896), lack of modularity, relatively high connectance (C=0.233), features encountered in mutualistic networks also. Betweenness centrality scores highlighted the keystone benefactor and beneficiary species, while betweenness centralization score (0.192) indicates the fact that there are several species sharing the dominant position in terms of interactions. The analysis of measurement data (seedlings’ and benefactor plants’ heights, distance from focal seedlings to nearest benefactor species and orientation) showed that there is common pattern in orientation (most of the benefactor species oriented toward South or South West) also in dimensional variability (MANOVA results).
Ecaterina Fodor, Ovidiu Haruta, Sorin Dorog
28.12.2018.
Research paper
Effects of substrate on germination and growth of Moringa oleifera Lam., Acacia mellifera (Vahl) Benth. and Zizyphus mauritiana Lam. seedlings
Despite the importance of forests and trees, Senegal is facing to the loss of forests and the decline of tree diversity and density. This study focuses on Moringa oleifera Lam., Acacia mellifera (Vahl) Benth. and Zizyphus mauritiana Lam. which constitute a significant source of food and economic value for the populations of Casamance. In addition to a germination test, a follow-up of seedlings on different substrates was carried out in the nursery, in order to determine the effect of substrates on some growth parameters (number of leaves, height, diameter and biomass).The substrates used were three potting mixtures of forest soil of Faidherbia albida (FSFA), Elaeis guineensis (FSEG) and Anacardium occidentale (FSAO) and sand with the following proportion: 1/3 sand + 2/3 forest soil). Based on species, the germination rate was higher for A. mellifera (84%) followed by M.oleifera (55%) and Z. mauritiana (50%). The germination rate was more important in FSFA and FSEG than in FSAO. For growth parameters, there was a significant effect (P<0.05) of substrate type on height, diameter, number of leaves and root biomass. In term of growth parameters, the seedling performed better in FSFA and FSEG than in FSAO. The species effect was also significant (P<0.05) with higher root biomass and diameter found in M. oleifera and height and number of leaves in A. mellifera. The most important total biomass was found in FSFA followed by FSEG. Comparing the fraction of biomass according to the part of the seedling, the stem and branch had the higher fraction of biomass for A. mellifera (43%) and M. oleifera (38%) and the leaves for Z. mauritiana (46%).However, thetype of substrate can affect seedling development and growth parameters and the better results were recorded in FSFA and FSEG substrates.
Ousmane Ndiaye, Arfang Ousmane Kemo Goudiaby, Antoine Sambou
28.12.2018.
Research paper
Screening mined-out indigenous mycorrhizal fungi for the rehabilitation of mine tailing areas in the Philippines
Arbuscular mycorrhizal fungi (AMF) play a significant role for mine tailing rehabilitation due to their sensitivity towards a range of soil pollutants. This beneficial biological agent can enhance plant tolerance to heavy metal contamination. This study screened indigenous AMF associated with growing indigenous ferns and grasses in the mine tailings for potential use in rehabilitating a 3-decade abandoned mined out area in Mogpog, Marinduque. Pterocarpus indicus Willd. (narra) was used as the host plant to establish mycorrhizal fungi association. Among the treatments, indigenous AMF associated with Ferns 1, 2 and 5 generally improved the height and shoot diameter of the narra seedlings and the effect was comparable with commercially available AMF inoculants, MYKOVAM® and MYKORICH®. The dry weight of the roots and nodules was consistently improved by indigenous AMF from Fern2 and Grass1, which had comparable effect with MYKOVAM® and MYKORICH®. Overall, the total seedling dry matter of narra seedlings was significantly stimulated by AMF, irrespective of isolates’ origin. The mycorrhizal root infection by AMF and number of spores in the soil were all high as compared with the uninoculated control counterpart. Lastly, AMF inoculation induced Cu retention in the roots of the seedlings. Thus the results imply that, the mined out indigenous AMF are potential agents to rehabilitate the abandoned mine tailings in the Philippines.
Nelly Siababa Aggangan, Angelbert D Cortes