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Issue 21, 2026

Online ISSN: 2466-4367

, Issue 21, (2026)

Published: 22.01.2026.

Open Access

This Special Issue of Reforesta, International Practices for Regenerating and Restoring Forest Trees by Seeding, brings together regional practice and research from North America, South America, Europe, Asia, and Oceania on using broadcast and direct seeding to create forest cover. It presents a significant range of seeding activities inclusive of species, forests, sites, and climates. Seeding has regained interest because of its scalability and cost-effectiveness, particularly for remote or inaccessible areas, sites with low productivity where planting seedling costs are prohibitive, or when aiming for a more natural forest structure. Technological innovations, particularly in seed treatments, automation, and precision seeding improve success of large-scale seed dispersal.

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31.12.2019.

Research paper

Acorn size influence on the quality of pedunculate oak (Quercus robur L.) one-year old seedlings

Pedunculate oak (Quercus robur L.) is one of the most important deciduous tree species in the Serbian and European forests. Different negative factors limit natural regeneration of pedunculate oak forests, so producing of high quality seedlings becomes imperative to ensure successful forest restoration. This research was conducted with to aim to determine whether acorn size is related to seedlings quality. Acorns were collected from the natural pedunculate oak forest and divided into two groups by their size. Acorns were sown in the spring into plastic containers after five months cold storage. Size of acorns was in the standard species range, though germination was low (< 20 %). Germination was more then double in group of large acorns in comparison to group of smaller acorns (19.2 %, respectively 8.6 %). Height and root collar diameter of produced seedlings were lower than seedlings produced in seedbeds in commercial nurseries in Serbia, but it was in range of container produced seedlings in some similar researches. Height to root collar diameter ratio and shoot to root ratio were considered as satisfactory for containerized seedlings. There was not strong correlation between acorn size and seedlings quality attributes.

Jovana R Devetaković, Marina Nonić, Bojan Prokić, Vladan Popović, Mirjana Šijačić-Nikolić

31.12.2019.

Review paper

Potassium fertilization in bareroot nurseries in the southern US: a review

This review covers most of the published literature on potassium (K) fertilization in bareroot seedbeds with the intent to concentrate on the southern United States. The timing and rates of K fertilization for bareroot seedlings are often based on logic and myths and, as a result, K recommendations vary considerably. Some recommend bareroot pine seedlings be fertilized with twice as much K as nitrogen (N) while others apply less than 100 kg ha-1. It was determined that several long-held claims about K fertilization are invalid. Nursery seedbeds do not need to contain four times as much available K as N and the belief that extra K fertilization will increase freeze tolerance or drought resistance of non-deficient seedlings is invalid. There are no data to support the claim that K fertilization increases root growth or assists in the formation of terminal buds. For sandy seedbeds, there is no need to apply K before sowing. Adding extra K during the fall does not increase seedling morphology or seedling performance when loblolly pine seedlings, at lifting, have more than 0.5% K in needles. A reduction of K fertilization can be achieved by reviewing foliar tests prior to K top-dressings.

David B. South

31.12.2019.

Review paper

Reforestation in Venezuela – current situation and future perspectives

The location of the Bolivarian Republic of Venezuela in the tropical region of northern South America, in the Northern Hemisphere, with a total area of 91.64 million ha, of which 49.6 million correspond to natural forests (54.12 % of the national territory). Venezuela shows a series of geographic advantages that allow a rapid growth and development of the trees in comparison with the temperate zones where the growth is extremely slow. However, the occurrence of deforestation processes has intensified in a sustained way in the recent history of the country.

In the period from 1920 to 2008, the loss of 26.43% of the forested area has been recorded. Likewise, in the period 1975-1988, deforestation was estimated at 216,000 ha year-1, equivalent to 2.9 million ha in 13 years (Catalan 1992). Deforestation has now increased to worrying levels and according to FAO estimates (2010), in Venezuela, a forested area of more than 288.000 ha year-1 is deforested.

In the middle of the 20th century several public and private institutions began an important effort to reforest areas of sandy soils of scarce depth on a bed of highly impermeable clay. With erosion processes generated as a result of strong winds that causes severe damage to the little existing vegetation. It was expected that with the passage of time this region could become a desert in the oriental plains of Venezuela. This is how the Maderas del Orinoco Companies (CVG - PROFORCA), the Venezuelan Corporation of Guayana (CVG), Smurfit Kappa Cartón of Venezuela, San Carlos Forestry Development, Masisa. Propulso, among others, started in different regions of the country the establishment of plantations for various purposes. For this they have used fast growing forest species such as: Pinus caribaea var hondurensis, Eucalyptus Urophylla, Acacia magnium, Caraipa llanorum, Tectonia grandis, Hebea brasiliens, Gmelina arborea, Erisma uncinatum, among others. At present, a reforested area of 650.000 ha has been reached. This has favored the protection of natural habitats by reducing the pressure for exploitation and use of the forests.

The legal framework established in the new National Constitution, approved in 1998, is based on decrees of 1825-1829 of the Liberator Simón Bolívar and classifies the territory in diverse forest areas establishing a modern environmental conservation policy. A historical summary and the current legislation with the classification that promises a rational management of the national territory is presented.

Francisco Visaez, Eduardo D. Greaves

28.06.2019.

Research paper

Seed germination of Crataegus monogyna – a species with a stony endocarp

The present work demonstrates the effects of moist cold stratification on seed dormancy breaking in Hawthorn (Crataegus monogyna Jacq.). We also examined the fruit and seed morphology. Mature and ripe fruits were collected, the pulp removed and the seeds (stones) left in the sun to dry for three days. Four temperature regimes viz. 4°C, 6.5°C (natural conditions where the fruits were harvested), 10°C and 20°C were used for stimulating seed germination under total darkness. For each treatment, there were four replicates with 50 seeds incubated in a plastic container between two layers of moist sand at 15%. At the end of the experiment, non-germinating seeds were tested for viability using Tetrazolium chloride (TZ).  After 4 months, the final germination (FGP) was expressed as a percentage of the total number of seeds in each treatment. The fruits of Hawthorn were 14.9 ± 0.73 mm long and 15.1 ± 0.84 mm in diameter and weigh 2.05 ± 0.28 g. The seeds were 8.29 ± 0.43 mm long and 6.75 ± 0.39 mm in diameter and weigh (0.25 ± 0.04) g. The thousand-fruit weight was 2,000 g and of the thousand-seeds weight was 280 g. The statistical analysis indicated significant effect (p < 0.0001) of treatment on seed germination. Dormancy in this species was broken most effectively by cold stratification at 4°C and under natural conditions with 76% and 67.5% of FGP, respectively. Most of the nongerminated seeds of C. monogyna were viable (dormant) as judged by TZ.

Abdenour Kheloufi, Lahouaria Mounia Mansouri, Cécile Vanbellinghen

10.07.2018.

Review paper

Cost of inoculating seedlings with Pisolithus tinctorius spores

Although the production of commercial products of vegetative Pt (Pisolithus tinctorius (Pers.) Coker & Couch) inoculums has ceased in North America due to a lack of demand by consumers, the number of products that contain Pt spores has increased. The quality, quantity and price of these products vary considerably. The cost of inoculating 1,000 tree seedlings with Pt basidiospores can vary from $0.45 to more than $30. The cost of treating with Pt spores is lowest when seedlings are inoculated in a container nursery using rates that are less than 0.4 mg per seedling. However, with some products the cost to treat 1,000 bareroot seedlings is greater than $500 when spores are applied in the planting hole. Three decades ago, 1 g of Pt spores could be purchased for $0.13 and now the price of 1 g can exceed $14. Although many research papers provide data on the biological response to inoculating seedlings with spores, few document the cost of inoculation. Commercial products that are marketed toward homeowners containing both ectomycorrhizal and endomycorrhizal spores are more expensive than products that contain only ectomycorrhizal spores. In situations where survival and growth of seedlings are not increased, the benefit/cost ratio will typically be less than one.

David B. South

28.12.2018.

Research paper

Alley cropping with strawberries: Two case-studies in Romania

The paper focuses on the use of alley cropping, an arable agroforestry system comprising rows of trees planted with a companion crop grown in the alleyways between the rows, in two sub-compartments (81B%: 0.75 ha, elevation 715 m asl; 81E: 3.4 ha, elevation 735 m asl) artificially regenerated at 1.80 m x 1.60 m spacing in the south-east of Transylvania (Romania) in 1997 (81B%) and 2003 (81E). The climate (Dfbx, specific to low and middle mountains) and soil (luvisol, of moderate fertility for pure or mixed Quercus petraea stands) are only moderately favourable to strawberry culture. The rows of tree species consists of Quercus rubra, Acer pseudoplatanus, Prunus avium (81B%), and Quercus petraea, Fagus sylvatica, Acer pseudoplatanus, and Larix decidua (81E). Strawberries (variety Elsanta,) were planted at 60 x 60 cm spacing in two pure rows in-between the tree rows as well as within the rows of planted trees. Both plantations were fenced, with manual topsoil hoeing up to four times a year, lighly fertilized (N, P, K complex) and chemically protected against browsing and pests. Strawberry rotation (six years, of which five years - two to six - with fruit production) was longer than the one (maximum three years) recommended in pure crops. Strawberry production reached the maximum level in years 3 or 4.

Under the local conditions, the alley cropping with strawberries had demonstrated to be a viable alternative to the 'classical' monocropping system in terms of (a) survival rate of plants (over 95 per cent), (b) initial height growth of plants (very quick, resulting from reduced competition with grasses and naturally regenerated forest trees such as pioneer species), (c) establishment of a new forest culture, the full forest cover being achieved immediately after the end of strawberry production, as well as (d) economics (early and relevant revenue from the crop).

Valeriu Norocel Nicolescu, Cornelia Hernea, Vilhelm Sandi

28.12.2018.

Research paper

Nurse plants and the regeneration niche of tree seedlings in wood-pastures from Western and North-Western Romania

Facilitation is a positive interaction demonstrated to be one of the important factors shaping the regeneration niche of trees, mostly under stressful conditions which is currently studied in the frame of complex ecological networks. The protection provided by benefactor plants for tree seedlings playing the role of beneficiaries is documented mainly in arid and semi-arid habitats or in situations where herbivores’ pressure constitutes the main stressful factor for tree regeneration. One of the iconic Transylvanian landscapes is the wood-pasture, also one of the oldest agro-forestry systems to which recent forest expansion in abandoned agricultural fields or pastures is added. The proposed work represents a preliminary investigation on the association between benefactor plants, mostly spiny shrubs (Rosa canina, Crataegus monogyna, Prunus spinosa as the most frequently encountered benefactors) and tree seedlings (Quercus spp., Tilia spp., Carpinus betulus, Fraxinus angustifolia, Pyrus pyraster as most frequently encountered beneficiaries), in four different locations from North-Western and Western Romania, wood-pastures, abandoned pastures and abandoned agricultural fields under the consideration that the main stressful factor is represented by livestock grazing. Bipartite, qualitative merged network was generated depicting the interaction between beneficiaries and benefactors. Commonly used metrics were calculated: connectivity, nestedness, modularity, betweenness centrality and centralization compared to similar facilitation networks presented in the literature. Facilitation network is characterized by high nestedness (N=0.896), lack of modularity, relatively high connectance (C=0.233), features encountered in mutualistic networks also. Betweenness centrality scores highlighted the keystone benefactor and beneficiary species, while betweenness centralization score (0.192) indicates the fact that there are several species sharing the dominant position in terms of interactions. The analysis of measurement data (seedlings’ and benefactor plants’ heights, distance from focal seedlings to nearest benefactor species and orientation) showed that there is common pattern in orientation (most of the benefactor species oriented toward South or South West) also in dimensional variability (MANOVA results).

Ecaterina Fodor, Ovidiu Haruta, Sorin Dorog

28.12.2018.

Research paper

Effects of substrate on germination and growth of Moringa oleifera Lam., Acacia mellifera (Vahl) Benth. and Zizyphus mauritiana Lam. seedlings

Despite the importance of forests and trees, Senegal is facing to the loss of forests and the decline of tree diversity and density. This study focuses on Moringa oleifera Lam., Acacia mellifera (Vahl) Benth. and Zizyphus mauritiana Lam. which constitute a significant source of food and economic value for the populations of Casamance. In addition to a germination test, a follow-up of seedlings on different substrates was carried out in the nursery, in order to determine the effect of substrates on some growth parameters (number of leaves, height, diameter and biomass).The substrates used were three potting mixtures of forest soil of Faidherbia albida (FSFA), Elaeis guineensis (FSEG) and Anacardium occidentale (FSAO) and sand with the following proportion: 1/3 sand + 2/3 forest soil). Based on species, the germination rate was higher for A. mellifera (84%) followed by M.oleifera (55%) and Z. mauritiana (50%). The germination rate was more important in FSFA and FSEG than in FSAO. For growth parameters, there was a significant effect (P<0.05) of substrate type on height, diameter, number of leaves and root biomass. In term of growth parameters, the seedling performed better in FSFA and FSEG than in FSAO. The species effect was also significant (P<0.05) with higher root biomass and diameter found in M. oleifera and height and number of leaves in A. mellifera. The most important total biomass was found in FSFA followed by FSEG. Comparing the fraction of biomass according to the part of the seedling, the stem and branch had the higher fraction of biomass for A. mellifera (43%) and M. oleifera (38%) and the leaves for Z. mauritiana (46%).However, thetype of substrate can affect seedling development and growth parameters and the better results were recorded in FSFA and FSEG substrates.

Ousmane Ndiaye, Arfang Ousmane Kemo Goudiaby, Antoine Sambou

28.12.2018.

Research paper

Screening mined-out indigenous mycorrhizal fungi for the rehabilitation of mine tailing areas in the Philippines

Arbuscular mycorrhizal fungi (AMF) play a significant role for mine tailing rehabilitation due to their sensitivity towards a range of soil pollutants. This beneficial biological agent can enhance plant tolerance to heavy metal contamination. This study screened indigenous AMF associated with growing indigenous ferns and grasses in the mine tailings for potential use in rehabilitating a 3-decade abandoned mined out area in Mogpog, Marinduque. Pterocarpus indicus Willd. (narra) was used as the host plant to establish mycorrhizal fungi association. Among the treatments, indigenous AMF associated with Ferns 1, 2 and 5 generally improved the height and shoot diameter of the narra seedlings and the effect was comparable with commercially available AMF inoculants, MYKOVAM® and MYKORICH®. The dry weight of the roots and nodules was consistently improved by indigenous AMF from Fern2 and Grass1, which had comparable effect with MYKOVAM® and MYKORICH®. Overall, the total seedling dry matter of narra seedlings was significantly stimulated by AMF, irrespective of isolates’ origin. The mycorrhizal root infection by AMF and number of spores in the soil were all high as compared with the uninoculated control counterpart. Lastly, AMF inoculation induced Cu retention in the roots of the seedlings. Thus the results imply that, the mined out indigenous AMF are potential agents to rehabilitate the abandoned mine tailings in the Philippines.

Nelly Siababa Aggangan, Angelbert D Cortes

28.12.2018.

Research paper

Mid- and long-term effects of stock type on the growth and yield of spruce seedlings in a non-herbicide scenario

Stock types used in reforestation projects can influence plantation success, as they determine the morphological attributes of the planted seedlings. They can also interact with silviculture treatments to influence early seedling survival and growth. As nurseries develop and produce new stock types in response to –and in interaction with– manager needs, research efforts must be pursued to validate early seedling performance and long-term growth and yields. In this context, we aimed to evaluate the main and interactive effects of mechanical site preparation and stock type on planted black (Picea mariana [Mill.] BSP) and white spruce (P. glauca [Moench.] Voss) seedling dimensions at 16-y, and estimate the long-term impact of stock type on the merchantable volume at rotation age for white spruce. We hence compared medium (200 cm3 root plug) and large (350 cm3 root plug) containerized seedlings, as well as large bare-root seedlings of both species, in a field experiment established in Quebec (Canada), where there is a ban on the use of chemical herbicides for vegetation management treatments. Our results confirm that there is a significant, although limited impact of stock type on the size of black and white spruce at the juvenile stage, when medium and large stock types are compared, but that these small differences have a negligible effect on the estimated merchantable volume produced at rotation age (60 years). Mechanical site preparation does not promote seedling growth on these rich sites with thin humus. Therefore, selection of a medium or larger stock type for reforestation projects and application of mechanical site preparation in ecosystems similar to the one studied here should be based on other considerations than growth and yield, such as seedling availability, production and planting costs, or operational constraints.

Nelson Thiffault, Charles Ward