Climate change is increasing the frequency and intensity of drought in the western USA. Evidence suggests that drought preconditioning of plants may improve the survival of planted seedlings under dry conditions through enhanced water uptake by roots, but the mechanisms underlying enhanced survival under drought remain unknown. We tested whether the vertical distribution of roots in root plug cross-sections varied with drought preconditioning and seed source. We subjected western larch (Larix occidentalis Nutt.) seedlings from eight different provenances to Low (50-65% gravimetric water content), Moderate (65-75%), and High (≥75%) watering regimes in a nursery. We then investigated dry root mass across four root-plug sections, including the taproot and three lateral root cross-sections (top 1/3rd, middle 1/3rd, and bottom 1/3rd of root plugs). We also tested for carry-over effect of drought preconditioning on the mass of egressed roots observed among cross-sections of potting soil in a 30-day potted study. Root plug mass varied significantly (P< 0.001) with watering regime, root plug cross-section, and an interaction between watering regime and cross-section. Overall, seedlings that received less water produced lateral root plug cross-sections of greater mass, which coincided with taproots of less mass. In contrast to findings from the root plug study, the distribution of egressed root mass among cross-sections of potting soil did not vary with drought preconditioning. This is the first study to assess seedling root growth in response to drought preconditioning in western larch with a focus on the distribution of root mass in root plugs and egressed root mass among soil cross-sections. We expect this work to facilitate future efforts to improve drought hardiness of western larch seedlings. Future improvement of western larch seedlings will require investigation into whether altered root plug mass distribution translates to improved seedling performance in outplanting trials.

Nursery seedlings with visual boron (B) deficiencies are rare, especially for broadleaf species but they may have occurred in conifer nurseries in Florida, Oregon and the UK. Factors favoring a deficiency include high soil pH, high soil calcium and low soil moisture (i.e. withholding irrigation). Symptoms of a boron deficiency in pine include dead terminals, resin exudation from buds, dark green foliage, and terminal needles with less than 3 μg g^-1 B. Chlorosis is an iron deficiency symptom but is not a boron deficiency symptom. At some nurseries (with more than 2% organic matter and more than 0.05 μg g^-1 B in irrigation water), seedlings do not have a hidden hunger for B. As a result, there are no published trials that demonstrate a positive growth response from adding boron to managed nursery soils (when seedbed density is not reduced by boron). This review highlights some of the past and current uses of B in nurseries with a focus on deficiency and toxicity effects.

Although the presence of root anatomical structures of young Pinus ponderosa seedlings grown in containers of contrasting volume (164 vs. 7000 cm³) was similar, seedlings reared 60 days in the large container had more vascular cambium although the xylem thickness was similar. In addition, seedlings in large containers had nearly twice as many resin ducts within the vascular cambium as their cohorts in small containers. Taproot length closely matched container depth. Though lateral root emission rates were similar between container sizes, large container seedlings had more than 2X the number of lateral roots as those from small containers. These differences in morphophysiological characteristics may be important to seedling establishment on sites that experience dry summer conditions, or for seedlings destined to drier, harsher sites. Further work to elucidate the ramifications of these morphophysiological differences on seedling establishment is warranted.

The Austrian pine (Pinus nigra Arnold) is a species often used in afforestation/reforestation programs in Serbia and region. Seedlings quality has a great role in outplanting survival and growth. Nursery cultural practice has a central influence on seedlings morphological attributes, so we tested seedlings quality from different seedbed densities and seedlings patterns and their outplanting success. Two-year-old bareroot seedlings were grown in very high densities (110 seedlings m^-1 in rows and 750 seedlings m^-2 across all seedbed space) and high densities (65 seedlings m^-1 in rows and 450 seedlings m^-2 across all seedbed space). Morphological attributes (root collar diameter - RCD, height - H, sturdiness coefficient - SQ, root length – Rl, dry weight of shoot - SDW and root - RDW, shoot to root ration - S:R and quality index - QI) of seedlings from different densities were tested in the nursery before outplanting and compare with seedlings survival and growth (root collar diameter – RCD1 and height – H1) one year after field growing. Seedlings from different densities had accepted RCD (>3 mm) for field planting and there were significant differences in their morphological attributes, except Rl and S:R. The best survival was recorded at seedlings from highest seedbed densities (93.33%), opposite with many previous researches. Seedlings grown across all seedbed space (450 seedlings per m^-2) keep their advantage in growth after first year, although survival was lowest (46.67%).

Deforestation in Ghana has led to a forest loss of almost 20% from 9,924,000 ha in 1990 to 7,986,000 ha today. To restore degraded lands, Forest Landscape Restoration has become a critical approach globally. This study was conducted in Ghana focusing on the examples of two forest landscape restoration projects in the Pamu Berekum Forest Reserve: 10-year-old mixed-stands of two to four native tree species and an exotic species stands, including Triplochiton scleroxylon, Terminalia ivorensis, Ceiba pentandra, Nauclea diderrichii and Cedrela odorata at Pamu Berekum 1 and 4-year-old Tectona grandis and 2-year-old Gmelina arborea monoculture stands at Pamu Berekum 2. Estimates of productivity in the restored forests are described, as well as the effects of the restoration on provision of ecosystem service and benefits obtained by local communities. Stand productivity was assessed as mean annual increment of diameter and height, biomass production, and standing volume. For ecosystem services, carbon stocks were calculated for the restored forests; other ecological benefits, as well as financial benefits, were obtained through interviews with fringe communities. The results indicate that FLR can be implemented successfully using different models provided that local communities are involved during the planning and implementation of interventions. When all stands were projected to 10 years, results show higher productivity in T. grandis (331.77 m³ ha^-1) and G. arborea stands (1,785.99 m³ ha^-1) compared to mixed stand (160.41 m³ ha^-1). The Gmelina arborea stand was more productive and had higher carbon stocks (1,350.10 Mg ha^-1) relative to the T. grandis stand (159.89 Mg ha^-1). Both restoration projects were found to deliver important benefits and ecosystem services at the local and national levels, including direct and indirect benefits. The results provide an example for forest/environmental managers on how FLR might be implemented to create multiple benefits at different levels from local communities to the national level. Thus, these results may be useful for guiding successful restoration activities within the context of the ongoing global Forest Landscape Restoration efforts.

Species complex Fusarium sambucinum was frequently isolated from ash stands in Montenegro. Previous researches revealed that it is dangerous pathogen for one-year-old seedlings. The aim of this research was to investigate the influence of temeperature on growth and morphological characteristics of morphotype isolated from one-year-old common ash (Fraxinus excelsior L.) seedlings in Montenegro. Growth of Fusarium sambucinum pure cultures on different temperatures was the fastest on 25°C while the absence of growth occurred on 32.5°C. Cultures on optimum temperature were white reddish, aerial and with a lot hyaline, curved multi septate conidia. This research covers basic ecological and morphological characteristics of Fusarium sambucinum strain responsible for high pathogenicity towards one-year-old common ash seedlings in Montenegro.

Investigation of different seed diseases of common ash has great significance due to occurrence of ash dieback across Europe caused by Hymenoscyphus fraxineus Baral, Queloz and Hosoya (T.Kowalski) and still not completely defined strategies about regeneration of this species. Species complex Diaporthe eres are familiar pathogens of common ash. During the monitoring of health condition of common ash in Montenegro Diaporthe eres was frequently isolated from common ash seeds. The aim of this research was to investigate temperature conditions that contribute to the growth of Diaporthe eres strains from Montenegro in infected seeds and histological characteristics of Diaporthe eres spread in seeds. Growth of Diaporthe eres pure cultures on tested temperatures showed the fastest growth on 25°C and the absence of growth on 35°C. Mechanical damage did not significantly contribute to occurrence of infection. Microscopic examination of heavy infected seeds showed that hyphae of D.eres were visible on outer parts of seed spreading inside the core. In this research we investigated some basic bio ecological characteristics of D. eres associated with decline of F. excelsior seed in Montenegro.

Norway spruce is one of the most commonly used species for new forest planting in Europe. It is planted in a large number of habitats, often without following the previous results in the success of afforestation. In order to improve Norway spruce afforestation, open field tests were established in which developmental phenophases are monitored. The use of planting material of different provenances, which had not previously been tested for habitat conditions, was often the cause of the decline of newly planted forests. Early budburst of Norway spruce causes losses due to the freezing of terminal buds. Norway spruce testing for different habitat conditions in Bosnia and Herzegovina (B&H) was conducted at two ecologically different localities: Srebrenica (eastern part of B&H) and Drinić (western part of B&H). During 3 years, the budburst on the seedlings originating from 6 populations (Han Pijesak 1, Han Pijesak 2, Foča, Olovo, Kneževo and Potoci) was monitored. The budburst dynamics was monitored in 2013, 2015 and 2016 and it was recorded for each seedling in two progeny tests. Seedlings from the Kneževo population budbursted the earliest. The seedlings would start budburst on different days of the year, depending partly on the temperature sums and their origin. The earliest budburst was recorded in 2013 (119^th day of the year in Srebrenica and 121^st day of the year in Drinić). During 2015 and 2016, the budburst started later (125^th day in Srebrenica and 129^th day in Drinić). Temperature cumulants indicate that a smaller sum of temperatures was required for the buburst in the Srebrenica test than in the Drinić test. However, the temperature sums did not clearly indicate the budburst pattern because they were different for each observed year, but the populations ranking was almost the same. This indicates the influence of some other variables on the budburst. The knowing of the data on the budburst dynamics are a prerequisite for a successful selection of starting populations from which planting material is produced and new forests are later planted. Population Kneževo had the earliest budburst but population Han Pijesak 2 had the latest budburst.

The carob tree (Ceratonia siliqua L.) is an important component of the Mediterranean vegetation and its cultivation is important environmentally and economically. It is also an interesting leguminous species for afforestation-reforestation. In this study, carob seeds collected in a representative area of the Mediterranean basin at the national park of Gouraya (Béjaïa, Algeria), were subjected to germination tests under Mediterranean seawater (SW) irrigation of different concentrations (0, 10, 30, 50 and 100% SW) for 15-day period. Before germination tests, a 20 min pre-treatment with 96% sulphuric acid was necessary to overcome seed coat dormancy which does not permit germination. Results showed that the seeds of C. siliqua were able to germinate at different seawater concentration, except for 50% SW and 100% SW which resulted in total inhibition of germination. The maximum number of C. siliqua seed germination of 100% FGP (final germination percentage) appeared at 0% SW and 10% SW. Only 35.5% of the seeds have germinated in 30% SW. Ungerminated seeds of C. siliqua from different SW treatments showed medium germination recovery (FGP_Recov) of 39.9% at 50% SW and low recovery of 18.2% at 100% SW when transferred to distilled water after 15 day-period. Seedlings length and seedling fresh and dry weight were significantly (P < 0.001) decreased with increasing SW concentrations. Seedling water content remained constant in 10% SW in comparison with the control, while it decreased very slightly in 30% SW. These findings may serve as useful information for C. siliqua habitat establishment and afforestation-reforestation programs in coastal sites and for exploiting seawater in the area.

The leaf area of plant leave scan be considered as an indicator of the plant growth conditions, and its variability is usually associated with adaptation and response to the changing environment. The aim of this study is to investigate the relation of the leaf area parameters on growth of the narrow-leaved ash 1+0 seedlings from the nursery trial and determining variability between the 18 half-sib lines. The seedlings were obtained from seeds collected from 18 maternal trees found in the area of the special nature reserve Upper Danube Region. Ten randomly selected 1+0 seedlings per half-sib line were selected for further study, and all leaves per seedling were collected, herbarized and scanned. The leaf area of each leaf (LACL) was measured using an open-source image processing program – ImageJ, and the total leaf area per seedling (TLA) was calculated. Also, the number of leaves per plant (NCL) as well as the number of leaflets within each imparipinnate compound leaf (NLCL) were counted. The results showed that differences between studied attributes were statistically significant among 18 selected half-sib lines (p < 0.05). The most variable parameter was the total leaf area per seedling (TLA; CV=68.98%), which is strongly correlated to the leaf area per each leaf (LACL; r=0.95) and the number of leaflets (NLCL; r=0.94). In this study, we confirmed that leaf parameters were highly correlated to the growth of one-year-old Fraxinus angustifolia seedlings.