Early pine root anatomy and primary and lateral root formation are affected by container size: implications in dry-summer climates

Main Article Content

Antonio Montagnoli
https://orcid.org/0000-0002-8921-0754
Donato Chiatante
Anastazija Dimitrova
Mattia Terzaghi
https://orcid.org/0000-0002-8803-7726
Jeremiah R. Pinto
R. Kasten Dumroese
https://orcid.org/0000-0002-2790-5286

Abstract

Although the presence of root anatomical structures of young Pinus ponderosa seedlings grown in containers of contrasting volume (164 vs. 7000 cm3) was similar, seedlings reared 60 days in the large container had more vascular cambium although the xylem thickness was similar. In addition, seedlings in large containers had nearly twice as many resin ducts within the vascular cambium as their cohorts in small containers. Taproot length closely matched container depth. Though lateral root emission rates were similar between container sizes, large container seedlings had more than 2X the number of lateral roots as those from small containers. These differences in morphophysiological characteristics may be important to seedling establishment on sites that experience dry summer conditions, or for seedlings destined to drier, harsher sites. Further work to elucidate the ramifications of these morphophysiological differences on seedling establishment is warranted.

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How to Cite
Montagnoli, Antonio, Donato Chiatante, Anastazija Dimitrova, Mattia Terzaghi, Jeremiah R. Pinto, and R. Kasten Dumroese. “Early Pine Root Anatomy and Primary and Lateral Root Formation Are Affected by Container Size: Implications in Dry-Summer Climates”. REFORESTA, no. 12 (January 5, 2022): 20-34. Accessed January 24, 2022. https://journal.reforestationchallenges.org/index.php/REFOR/article/view/147.
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Articles
Author Biography

Antonio Montagnoli, Department of Biotechnology and Life Science, University of Insubria, Via Dunant, 3 – 21100 Varese, Italy.

Antonio’s work focusses upon the morpho-physiological responses of vegetation to environmental and anthropogenic drivers such as soil erosion, landslide, rainfall and forest management. In particular he studies the variation of fine roots dynamics during these responses at both single-tree and community level. His research group is interested in a number of topics such as LED-light influence on plant growth, plant phenotyping methods to improve efficiency in filed applied science, root system soil strengthening, and vegetation analysis trough remote sensing. His research activity is documented by peer-review scientific publications, posters- and oral-presentations in international meetings. After graduation in Environmental Science at University of Molise he obtained a PhD in Environmental Botany at University of Insubria where at present holds a staff member position in the Department of Biotechnologies and Life Science of University of Insubria (Varese – Italy).

References

Anderegg WRL, Hicke JA, Fisher RA, Allen CD, Aukema J, Bentz B, Sharon Hood S, Lichstein JW, Macalady AK, McDowell N, PanY, Raffa K, Sala A, Shaw JD, Stephenson NL, Tague C, Zeppel M (2015) Tree mortality from drought, insects, and their interactions in a changing climate. New Phytol 208: 674-683. https://doi.org/10.1111/nph.13477

Andivia E, Villar-Salvador P, Oliet JA, Puértolas J, Dumroese RK, Ivetić V, Molina-Venegas R, Arellano EC, Li G, Ovalle JF (2021) Climate and species stress tolerance modulate the higher survival of large seedlings in forest restoration worldwide. Ecol Appl 31: e02394. https://doi.org/10.1002/eap.2394

Aphalo P, Rikala R (2003) Field performance of silver-birch planting-stock grown at different spacing and in containers of different volume. New Forest 25: 93-108. https://doi.org/10.1023/A:1022618810937

Boucher D, Lavallée R, Mauffette Y (2001) Biological performance of the white pine weevil in relation to the anatomy of the resin canal system of four different host species. Can J Forest Res 31:2035-2041. https://doi.org/10.1139/x01-135

Bowden R (1993) Stock type selection in British Columbia. In: Huber R (ed), Proceedings of the 13th annual meeting of the Forest Nursery Association of British Columbia, Courtenay, British Columbia, pp 17-20.

Carlson LW, Endean F (1976) The effect of rooting volume and container configuration on the early growth of white spruce seedlings. Can J Forest Res 6:221-224. https://doi.org/10.1139/x76-027

Carlson WC, Larson MM (1977) Changes in auxin and cytokinin activity in roots of red oak, Quercus rubra, seedlings during lateral root formation. Physiol Plant 41: 162-166. https://doi.org/10.1111/j.1399-3054.1977.tb05551.x

Chirino E, Vilagrosa A, Hernandez EI, Matos A, Vallejo VR (2008) Effects of a deep container on morpho-functional characteristics and root colonization in Quercus suber L. seedlings for reforestation in Mediterranean climate. Forest Ecol Manag 256: 779-785. https://doi.org/10.1016/j.foreco.2008.05.035

Dale VH, Joyce LA, McNulty S, Neilson RP, Ayres MP, Flannigan MD, Hanson PJ, Irland LC, Lugo AE, Peterson CJ, Simberloff D, Swanson FJ, Stocks BJ, Wotton BM (2001) Climate change and forest disturbances: climate change can affect forests by altering the frequency, intensity, duration, and timing of fire, drought, introduced species, insect and pathogen outbreaks, hurricanes, windstorms, ice storms, or landslides. BioScience 51: 723-734. https://doi.org/10.1641/0006-3568(2001)051[0723:CCAFD]2.0.CO;2

Davis SD, Sperry JS, Hacke UG (1999) The relationship between xylem conduit diameter and cavitation caused by freezing. Am J Bot 86: 1367-1372. https://doi.org/10.2307/2656919

Dumroese RK, Davis AS, Jacobs DF (2011a) Nursery response of Acacia koa seedlings to container size, irrigation method, and fertilization rate. J Plant Nutr 34: 877-887. https://doi.org/10.1080/01904167.2011.544356

Dumroese RK, Page-Dumroese DS, Brown RE (2011b) Allometry, nitrogen status, and carbon stable isotope composition of Pinus ponderosa seedlings in two growing media with contrasting nursery irrigation regimes. Can J Forest Res 41:1091–1101. https://doi.org/10.1139/x11-017

Dumroese RK, Sung S-JS, Pinto JR, Ross-Davis A, Scott AD (2013) Morphology, gas exchange, and chlorophyll content of longleaf pine seedlings in response to rooting volume, copper root pruning, and nitrogen supply in a container nursery. New Forest 44: 881-897. https://doi.org/10.1007/s11056-013-9377-5

Dumroese RK, Montville ME, Pinto JR (2015) Using container weights to determine irrigation needs: a simple method. Native Plants J 16: 67-71. https://doi.org/10.3368/npj.16.1.67

Dumroese RK, Landis TD, Pinto JR, Haase DL, Wilkinson KM, Davis AS (2016) Meeting forest restoration challenges: using the Target Plant Concept. Reforesta 1:37-52. https://doi.org/10.21750/REFOR.1.03.3

Dumroese RK, Terzaghi M, Chiatante D, Scippa GS, Lasserre B, Montagnoli A (2019) Functional traits of Pinus ponderosa coarse roots in response to slope conditions. Front Plant Sci 10:947. https://doi.org/10.3389/fpls.2019.00947

Esteban LG, Martín JA, de Palacios P, Fernández (2012) Influence of region of provenance and climate factors on wood anatomical traits of Pinus nigra Arn. subsp. salzmannii. Eur J Forest Res 131: 633-645. https://doi.org/10.1007/s10342-011-0537-x

Grossnickle SC (2005) Importance of root growth in overcoming planting stress. New Forest 30: 273-294. https://doi.org/10.1007/s11056-004-8303-2

Grossnickle SC (2012) Why seedlings survive: influence of plant attributes. New Forest 43: 711-738. https://doi.org/10.1007/s11056-012-9336-6

Grossnickle SC, MacDonald JE (2018) Seedling quality: history, application, and plant attributes. Forests 9: 283. https://doi.org/10.3390/f9050283

Hasse DL, Davis AS (2017) Developing and supporting quality nursery facilities and staff are necessary to meet global forest and landscape restoration needs. Reforesta 4: 69-93. https://doi.org/10.21750/REFOR.4.06.45

Haywood JD, Sung SS, Sayer MAS (2012) Copper root pruning and container cavity size influence longleaf pine growth through five growing seasons. South J Appl Forest 36: 146-151. https://doi.org/10.5849/sjaf.10-051

Herms DA, Mattson WJ (1992) The dilemma of plants: to grow or defend. Q Rev Biol 67: 283-335. https://www.jstor.org/stable/2830650

Hood S, Sala A (2015) Ponderosa pine resin defenses and growth: metrics matter. Tree Physiol 35: 1223-1235. https://doi.org/10.1093/treephys/tpv098

Jacobs DF, Salifu KF, Seifert JR (2005) Relative contribution of initial root and shoot morphology in predicting field performance of hardwood seedlings. New Forest 30: 235-251. https://doi.org/10.1007/s11056-005-5419-y

Kane JM, Kolb TE (2010) Importance of resin ducts in reducing ponderosa pine mortality from bark beetle attack. Oecologia 164:601‒609. https://doi.org/10.1007/s00442-010-1683-4

Kavanagh KL, Zaerr JB (1997) Xylem cavitation and loss of hydraulic conductance in western hemlock following planting. Tree Physiol 17: 59-63. https://doi.org/10.1093/treephys/17.1.59

Keyes CR, Maguire DA, Tappeiner JC (2007) Observed dynamics of ponderosa pine (Pinus ponderosa var. ponderosa Dougl. ex Laws.) seedling recruitment in the Cascade Range, USA. New Forest 34: 95-105. https://doi.org/10.1007/s11056-007-9041-z.

Kolb PF, Robberecht, R (1996a) Pinus ponderosa seedling establishment and the influence of competition with the bunchgrass Agropyron spicatum. Int J Plant Sci 157: 509-515. http://www.jstor.org/stable/2475256

Kolb PF, Robberecht R (1996b) High temperature and drought stress effects on survival of Pinus ponderosa seedlings. Tree Physiol 16: 665-672.https://doi.org/10.1093/treephys/16.8.665

Kolb T, Dixit A, Burney O (2019) Challenges and opportunities for maintaining ponderosa pine forests in the southwestern United States. Tree Planters’ Notes 62(1&2): 104-112.

Korb JE, Fornwalt PJ, Stevens-Rumann CS (2019) What drives ponderosa pine regeneration following wildfire in the western United States? Forest Ecol Manag 454: 117663. https://doi.org/10.1016/j.foreco.2019.117663

Kurz WA, Dymond CC, Stinson G, Rampley GJ, Neilson ET, Carroll AL, Ebata T, Safranyik L (2008) Mountain pine beetle and forest carbon feedback to climate change. Nature 452: 987-990. https://doi.org/10.1038/nature06777

Landis TD, Tinus RW, McDonald SE, Barnett JP (1990) Containers and growing media, vol. 2. The container tree nursery manual. Agriculture Handbook 674. US Department of Agriculture Forest Service, Washington, DC. 88 p.

Landis TD, Steinfeld DE, Dumroese RK (2010) Native plant containers for restoration projects. Native Plants J 11:341-348. https://doi.org/10.2979/NPJ.2010.11.3.341

Larsen HS, South DB, Boyer JM (1986) Root growth potential, seedling morphology and bud dormancy correlate with survival of loblolly pine seedlings planted in December in Alabama. Tree Physiol 1: 253-263. https://doi.org/10.1093/treephys/1.3.253

Larson MM (1963) Initial root development of ponderosa pine seedlings as related to germination date and size of seed. Forest Sci 9: 456-460. https://doi.org/10.1093/forestscience/9.4.456

Lombardero M, Ayres M, Lorio Jr P, Ruel J (2000) Environmental effects on constitutive and inducible resin defences of Pinus taeda. Ecol Lett 3: 329-339. https://doi.org/10.1046/j.1461-0248.2000.00163.x

Mariotti B, Maltoni A, Jacobs DF, Tani A (2015) Container effects on growth and biomass allocation in Quercus robur and Juglans regia seedlings. Scand J Forest Res 30: 401-415. https://doi.org/10.1080/02827581.2015.1023352

Montagnoli A, Dumroese RK, Terzaghi M, Onelli E, Scippa GS, Chiatante D (2019) Seasonality of fine root dynamics and activity of root and shoot vascular cambium in a Quercus ilex L. forest (Italy). Forest Ecol Manag 431: 26-34. https://doi.org/10.1016/j.foreco.2018.06.044

Montagnoli A, Lasserre B, Sferra G, Chiatante D, Scippa GS, Terzaghi M, Dumroese RK (2020) Formation of annual ring eccentricity in coarse roots within the root cage of Pinus ponderosa growing on slopes. Plants 9: 181. https://doi.org/10.3390/plants9020181

Moreira X, Zas R, Solla A, Sampedro L (2015) Differentiation of persistent anatomical defensive structures is costly and determined by nutrient availability and genetic growth-defence constraints. Tree Physiol 35: 112-123. https://doi.org/10.1093/treephys/tpu106

Nelson WR (1989) Root pruning can influence first order lateral root development of containerised plants. Comb Proc Int Plant Prop Soc 49: 96-103.

Oliet JA, Jacobs DF (2012) Restoring forests: advances in techniques and theory. New Forest 3: 535-541. https://doi.org/10.1007/s11056-012-9354-4

Oliet JA, Planelles R, Artero F, Valverde R, Jacobs DF, Segura ML (2009) Field performance of Pinus halepensis planted in Mediterranean arid conditions: relative influence of seedling morphology and mineral nutrition. New Forest 37: 313-331. https://doi.org/10.1007/s11056-008-9126-3

Oliver WW, Ryker RA (1990) Pinus ponderosa Dougl. ex Laws. Ponderosa pine. Pinaceae – pine family. In: Burns RM, Honkala BH (tech cords) Silvics of North America: 1. Conifers. Agriculture Handbook 654. US Department of Agriculture, Forest Service, Washington, DC.

O'Neill GA, Aitken SN, King JN, Alfaro RI (2002) Geographic variation in resin canal defenses in seedlings from the Sitka spruce × white spruce introgression zone. Can J Forest Res 32: 390-400. https://doi.org/10.1139/x01-206

Owen SM, Sieg CH, Fulé PZ, Gehring CA, Baggett LS, Iniguez JM, Fornwalt PJ, Battaglia MA (2020) Persistent effects of fire severity on ponderosa pine regeneration niches and seedling growth. Forest Ecol Manag 477: 118502. https://doi.org/10.1016/j.foreco.2020.118502

Pemán J, Voltas J, Gil Pelegrin E (2006) Morphological and functional variability in the root system of Quercus ilex L. subject to confinement: consequences for afforestation. Ann Forest Sci 63: 425-430. https://doi.org/10.1051/forest:2006022

Pinto JR, Marshall JD, Dumroese RK, Davis AS, Cobos DR (2011) Establishment and growth of container seedlings for reforestation: a function of stocktype and edaphic conditions. Forest Ecol Manag 261: 1876-1884. https://doi.org/10.1016/j.foreco.2011.02.010

Pinto JR, Marshall JD, Dumroese RK, Davis AS, Cobos DR (2012) Photosynthetic response, carbon isotopic composition, survival, and growth of three stock types under water stress enhanced by vegetative competition. Can J Forest Res 42: 333-344. https://doi.org/10.1139/x11-189

Pinto JR, Marshall JD, Dumroese RK, Davis AS, Cobos DR (2016) Seedling establishment and physiological responses to temporal and spatial soil moisture changes. New Forest 47: 223-241. https://doi.org/10.1007/s11056-015-9511-7

Pittermann J, Sperry J (2003) Tracheid diameter is the key trait determining the extent of freezing-induced embolism in conifers. Tree Physiol 23: 907-914. https://doi.org/10.1093/treephys/23.13.907

Pureswaran DS, Roques A, Battisti A (2018) Forest insects and climate change. Curr Forest Rep 4: 35-50. https://doi.org/10.1007/s40725-018-0075-6

Rehfeld GE, Jaquish BC, Sáenz-Romero C, Joyce DG, Leites LP, St Clair JB, López-Upton J (2014) Comparative genetic responses to climate in the varieties of Pinus ponderosa and Pseudotsuga menziesii: reforestation. Forest Ecol Manag 324: 147-157.

https://doi.org/10.1016/j.foreco.2014.02.040

Ritchie GA, Landis TD, Dumroese RK, Haase DL (2010) Chapter 2: Assessing plant quality. In: Landis TD, Dumroese RK, Haase DL, The container tree nursery manual, vol 7: seedling processing, storage, and outplanting. Agriculture Handbook 674. US Department of Agriculture, Forest Service, Washington, DC, pp 17-81.

Rodman KC, Veblen TT, Battaglia MA, Chambers ME, Fornwalt PJ, Holden ZA, Kolb TE, Ouzts JR, Rother MT (2020) A changing climate is snuffing out post-fire recovery in montane forests. Global Ecol Biogeogr 29: 2039-2051. https://doi.org/10.1111/geb.13174

Rodríguez-García A, López R, Martín JA, Pinillos F, Gil L (2014) Resin yield in Pinus pinaster is related to tree dendrometry, stand density and tapping-induced systemic changes in xylem anatomy. Forest Ecol Manag 313: 47-54. https://doi.org/10.1016/j.foreco.2013.10.038.

Romero AE, Ryder J, Fisher JT, Mexal JG (1986) Root system modification of container stock for arid land plantings. Forest Ecol Manag 16: 281-290. https://doi.org/10.1016/0378-1127(86)90028-9

Sampedro L, Moreira X, Zas R (2011) Costs of constitutive and herbivore-induced chemical defences in pine trees emerge only under low nutrient availability. J Ecol 99: 818-827. https://doi.org/10.1111/j.1365-2745.2011.01814.x

Seidl R, Thom D, Kautz M, Martin-Benito D, Peltoniemi M, Vacchiano G, Wild J, Ascoli D, Petr M, Honkaniemi J, Lexer MJ, Trotsiuk V, Mairota P, Svoboda M, Fabrika M, Nagel TA, Reyer CPO (2017) Forest disturbances under climate change. Nat Clim Chang 76: 395-402. https://doi.org/10.1038/nclimate3303

South DB, Shelton J, Enebak SA (2001) Geotropic lateral roots of container-grown longleaf pine seedlings. Native Plant J 2: 126-130. https://doi.org/10.3368/npj.2.2.126

South DB, Harris SW, Barnett JP, Hainds MJ, Gjerstad DH (2005) Effect of container type and seedling size on survival and early height growth of Pinus palustris seedlings in Alabama, U.S.A. Forest Ecol Manag 204: 385-398. https://doi.org/10.1016/j.foreco.2004.09.016

Sturrock RN, Frankel SJ, Brown AV, Hennon PE, Kliejunas JT, Lewis KJ, Worrall JJ, Woods AJ (2011) Climate change and forest diseases. Plant Pathol 60: 133-149. https://doi.org/10.1111/j.1365-3059.2010.02406.x

Sun Q, Dumroese RK, Liu Y (2018) Container volume and subirrigation schedule influence Quercus valiabilis seedling growth and nutrient status in the nursery and field. Scand J Forest Res 33:560‒567. https://doi.org/10.1080/02827581.2018.1444787

Sutton RF (1980) Root system morphogenesis. New Zeal J Forest Sci 10: 264-292.

Urza AK, Weisberg PJ, Dilts T (2020) Evidence of widespread topoclimatic limitation for lower treelines of the Intermountain West, United States. Ecol Appl 30(07): e02158. https://doi.org/10.1002/eap.2158

Vázquez-González C, López-Goldar X, Zas R, Sampedro L (2019) Neutral and climate-driven adaptive processes contribute to explain population variation in resin duct traits in a Mediterranean pine species. Front Plant Sci 10: 1613. https://doi.org/10.3389/fpls.2019.01613

Vázquez-González C, Zas R, Erbilgin N, Ferrenberg S, Rozas V, Sampedro L (2020) Resin ducts as resistance traits in conifers: linking dendrochronology and resin-based defences. Tree Physiol 40: 1313-1326. https://doi.org/10.1093/treephys/tpaa064

Villar-Salvador P, Puértolas J, Peñuelas JL (2010) Assessing morphological and physiological plant quality for Mediterranean woodland restoration projects. In: Bautista S, Aronson J, Ramón Vallejo V (eds) Land restoration to combat desertification: innovative approaches, quality control and project evaluation. Fundación Centro de Estudios Ambientales del Mediterráneo (CEAM), Valencia, Spain. pp 103-120.

Villar-Salvador P, Puertolas J, Cuesta B, Peñuelas JL, Uscola M, Heredia-Guerrero N, Rey Benaya JM (2012) Increase in size and nitrogen concentration enhances seedling survival in Mediterranean plantations. Insights from an ecophysiological conceptual model of plant survival. New Forest 43: 755-770. https://doi.org/10.1007/s11056-012-9328-6

Wakeley PC (1935) Artificial reforestation in the southern pine region. Technical Bulletin 492. US Department of Agriculture, Forest Service, Washington, DC. 115 p.

Warren JM, Meinzer FC, Brooks JR, Domec JC (2005) Vertical stratification of soil water storage and release dynamics in Pacific Northwest coniferous forests. Agric Forest Meteorol 130: 39-58. https://doi.org/ 10.1016/j.agrformet.2005.01.004

Wenny DL (1995) Growing conservation seedlings by the square foot: making it pay. In: Landis TD, Cregg B (tech coords) National proceedings, forest and conservation nursery associations. General Technical Report PNW-GTR-365. US Department of Agriculture, Forest Service, Pacific Northwest Experiment Station, Portland, OR. pp 56-59.

Werker A, Fahn A (1969) Resin ducts of Pinus halepensis Mill.—Their structure, development and pattern of arrangement. Bot J Linn Soc 62: 379-411. https://doi.org/10.1111/j.1095-8339.1969.tb01974.x

Williams HM, South DB, Webb A (1988) Effects of fall irrigation on morphology and root growth potential of loblolly pine seedlings growing in sand. S Afr Forest J 147: 1-5. https://doi.org/10.1080/00382167.1988.9628963

Williams MI, Dumroese RK (2013) Preparing for climate change: forestry and assisted migration. J Forest 111: 287-297. https://doi.org/10.5849/jof.13-016

Yang M, Défossez P, Danjon F, Dupont S, Fourcaud T (2017) Which root architectural elements contribute the best to anchorage of Pinus species? Insights from in silico experiments. Plant Soil 411: 275-291. https://doi.org/10.1007/s11104-016-2992-0

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