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Lethals can be estimated from doubled haploid <i>Larix decidua </i>female gametophytes

Claire Williams ,
Claire Williams
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Patrick Von Aderkas
Patrick Von Aderkas
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Published: 01.12.2024.

Volume 0, Issue 19 (2025)

pp. 27-34;

https://doi.org/10.21750/refor.19.03.124

Abstract

We present a novel way of estimating embryo lethals using doubled haploid (dihaploid or DH) female gametophytes grown in tissue culture. This DH-based lethals estimate is hypothesized to be lower than the more common method where lethals are estimated from one generation of selfing. The chief reasoning is the early mortality spike observed for selfed embryos of larch and other genera in the Pinaceae. This early mortality spike occurs at the stage when diploid embryos embed into haploid female gametophyte tissue. However, this stage is missing for DH embryos which grow in a petri dish thus the DH lethals estimate is hypothesized to be lower compared to seed counts. This DH estimate is based on 20,000 haploid female gametophytes cultures from which two surviving diploidized embryos were recovered. Lethals based on diploidized haploids (DH) have not been reported for a conifer previously because, aside from larch, only a few conifer species continue female gametophyte development in absence of pollen. Our chief finding is that, contrary to our hypothesis, the DH-based lethals estimate was similar to lethals estimated obtained from selfed seeds in published studies. Thus doubling haploid female gametophytes can be used to estimate embryo lethals when diploidization is conducted on a massive scale.

References

Bonga, J. M., von Aderkas, P., & James, D. (1988). Potential Application of Haploid Cultures of Tree Species. In Genetic Manipulation of Woody Plants (pp. 57–77). https://doi.org/10.1007/978-1-4613-1661-9_4
Brandvain, Y., Harkness, A., & Pyhäjärvi, T. (2023). Reproductive Compensation and Selection among Viable Embryos Drive the Evolution of Polyembryony. The American Naturalist, 201(5), 694–711. https://doi.org/10.1086/723454
Brandvain, Y., Thomson, L., & Pyhäjärvi, T. (2024). Early-acting inbreeding depression can evolve as an inbreeding avoidance mechanism. Proceedings of the Royal Society B: Biological Sciences, 291(2018). https://doi.org/10.1098/rspb.2023.2467
Ebling, B. (1962). REVIEW. Language Learning, 12(4), 313–318. https://doi.org/10.1111/j.1467-1770.1962.tb01268.x
Haig, D., & Wilczek, A. (2006). Sexual conflict and the alternation of haploid and diploid generations. Philosophical Transactions of the Royal Society B: Biological Sciences, 361(1466), 335–343. https://doi.org/10.1098/rstb.2005.1794

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