In this paper the variability of morphological (needle length and needle width) and anatomical (needle thickness, resin ducts width, epidermis thickness, hypodermis height and number of the hypodermis layers) properties of black pine (Pinus nigra J.F. Arnold) needles were studied at the intra- and inter-population levels. Two mountains in Serbia, Jastrebac and Goč, were selected as experimental plots. Three Pinus nigra trees were selected from both localities (6 in total). Obtained results showed that trees on Goč had bigger dimensions of the following elements: needle length, slightly wider needle width, epidermis thickness, hypodermis height, number of hypodermis layers and needle thickness. On the other hand, resin ducts were only slightly wider by the trees from Jastrebac. The results of the analysis of variance showed that variation between studied populations, as well as variation between trees within populations was statistically significant for all needle traits except resin ducts width and hypodermis height.
Apple, M., Tiekotter, K., Snow, M., Young, J., Soeldner, A., Phillips, D., Tingey, D., & Bond, B. J. (2002). Needle anatomy changes with increasing tree age in Douglas-fir. Tree Physiology, 22(2–3), 129–136. https://doi.org/10.1093/treephys/22.2-3.129
Baltas, E. (2007). Spatial distribution of climatic indices in northern Greece. Meteorological Applications, 14(1), 69–78. https://doi.org/10.1002/met.7
Cvjetićanin, R., & Perović, M. (2010). Praktikum iz dendrologije. 41–42.
Egorova, N., & Kulagin, A. (2007). Features of the leaves structure of forest formed species in technogenic conditions. Samara Luka, 16, 463–476.
Grill, D., Tausz, M., Pöllinger, U. t. e., Jiménez, M. S., & Morales, D. (2004). Effects of drought on needle anatomy of Pinus canariensis. Flora - Morphology, Distribution, Functional Ecology of Plants, 199(2), 85–89. https://doi.org/10.1078/0367-2530-00137
Jankowski, A., Wyka, T. P., Żytkowiak, R., Nihlgård, B., Reich, P. B., & Oleksyn, J. (2017). Cold adaptation drives variability in needle structure and anatomy in <scp>P</scp>inus sylvestris L. along a 1,900 km temperate–boreal transect. Functional Ecology, 31(12), 2212–2223. https://doi.org/10.1111/1365-2435.12946
Jokanović, D. (2021). Praktikum iz lekovitog bilja. 95–97.
Jovanović, B. (1991). Dendrologija. 129–136.
Liber, Z., Nikolić, T., & Mitić, B. (n.d.). Intra- and interpopulation relationships and taxonomic status of Pinus nigra Arnold in Croatia according to morphology and anatomy of needles. Acta Societatis Botanicorum Poloniae, 71(2), 141–147. https://doi.org/10.5586/asbp.2002.016
López, R., Climent, J., & Gil, L. (2008). From desert to cloud forest: the non-trivial phenotypic variation of Canary Island pine needles. Trees, 22(6), 843–849. https://doi.org/10.1007/s00468-008-0245-4
LUOMALA, E., LAITINEN, K., SUTINEN, S., KELLOMÄKI, S., & VAPAAVUORI, E. (2005). Stomatal density, anatomy and nutrient concentrations of Scots pine needles are affected by elevated CO2 and temperature. Plant, Cell & Environment, 28(6), 733–749. https://doi.org/10.1111/j.1365-3040.2005.01319.x
Niinemets, U., Ellsworth, D. S., Lukjanova, A., & Tobias, M. (2001). Site fertility and the morphological and photosynthetic acclimation of Pinus sylvestris needles to light. Tree Physiology, 21(17), 1231–1244. https://doi.org/10.1093/treephys/21.17.1231
Nikolic, B., Mitic, Z., Bojovic, S., Matevski, V., Krivosej, Z., & Marin, P. (2019). Variability of needle morpho-anatomy of natural Pinus heldreichii populations from Scardo-Pindic mountains. Genetika, 51(3), 1175–1184. https://doi.org/10.2298/GENSR1903175N
Pensa, M., Aalto, T., & Jalkanen, R. (2004). Variation in needle-trace diameter in respect of needle morphology in five conifer species. Trees - Structure and Function, 18(3), 307–311. https://doi.org/10.1007/s00468-003-0307-6
Popović, V., Šešlija Jovanović, D., Miljković, D., Vasiljević, A., Jovanović, S., Rakonjac, L., & Lučić, A. (2022). Prostorna varijabilnost morfoloških obilježja iglica populacija jele (Abies alba Mill.) na Balkanskom poluotoku u odnosu na klimatske čimbenike. Šumarski List, 146(7–8), 309–317. https://doi.org/10.31298/sl.146.7-8.3
Schoettle, A. W., & Rochelle, S. G. (2000). Morphological variation of Pinus flexilis (Pinaceae), a bird‐dispersed pine, across a range of elevations. American Journal of Botany, 87(12), 1797–1806. https://doi.org/10.2307/2656832
TIBCO Software Inc. (2017).
Urbaniak, L., Karliński, L., & Popielarz, R. (n.d.). Variation of morphological needle characters of Scots pine (Pinus sylvestris L.) populations in different habitats. Acta Societatis Botanicorum Poloniae, 72(1), 37–44. https://doi.org/10.5586/asbp.2003.005
Vilotić, D. (2000). Uporedna anatomija drveta. 60–69.
(n.d.). Crni bor na stijenama – varijabilnost i mogućnost korišćenja.
(2013). Climate of the Greater Carpathian Region.
