The Ecological and histological investigation of species complex Diaporthe eres on Fraxinus excelsior seed from Montenegro

Aleksandar Vemić

doi:10.21750/REFOR.11.02.90

Authors

Aleksandar Vemić a:1:{s:5:"en_US";s:65:"Faculty of Forestry – University of Belgrade, Belgrade, Serbia";}

DOI:

https://doi.org/10.21750/REFOR.11.02.90

Keywords:

Diaporthe eres, Fraxinus excelsior, Seed, Bio ecology

Abstract

Investigation of different seed diseases of common ash has great significance due to occurrence of ash dieback across Europe caused by Hymenoscyphus fraxineus Baral, Queloz and Hosoya (T.Kowalski) and still not completely defined strategies about regeneration of this species. Species complex Diaporthe eres are familiar pathogens of common ash. During the monitoring of health condition of common ash in Montenegro Diaporthe eres was frequently isolated from common ash seeds. The aim of this research was to investigate temperature conditions that contribute to the growth of Diaporthe eres strains from Montenegro in infected seeds and histological characteristics of Diaporthe eres spread in seeds. Growth of Diaporthe eres pure cultures on tested temperatures showed the fastest growth on 25°C and the absence of growth on 35°C. Mechanical damage did not significantly contribute to occurrence of infection. Microscopic examination of heavy infected seeds showed that hyphae of D.eres were visible on outer parts of seed spreading inside the core. In this research we investigated some basic bio ecological characteristics of D. eres associated with decline of F. excelsior seed in Montenegro.

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