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Issue 21, 2026

Online ISSN: 2466-4367

, Issue 21, (2026)

Published: 22.01.2026.

Open Access

This Special Issue of Reforesta, International Practices for Regenerating and Restoring Forest Trees by Seeding, brings together regional practice and research from North America, South America, Europe, Asia, and Oceania on using broadcast and direct seeding to create forest cover. It presents a significant range of seeding activities inclusive of species, forests, sites, and climates. Seeding has regained interest because of its scalability and cost-effectiveness, particularly for remote or inaccessible areas, sites with low productivity where planting seedling costs are prohibitive, or when aiming for a more natural forest structure. Technological innovations, particularly in seed treatments, automation, and precision seeding improve success of large-scale seed dispersal.

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01.12.2019.

Research paper

Forest restoration on the former industrial land of Sulphur quarry in the Ukrainian Roztochya

This paper adds to the evidence base in the discussion to what extent woodland development can be a means to restoring the land affected by mining. We investigate the formation of vegetation communities in sites on a former Sulphur quarry in the Roztochya district of the Lviv region in Ukraine to answer the research questions: How can the formation of vegetation cover affect the disturbed sites and what knowledge can be used in restoration practices elsewhere? We perform an experiment examining which types of vegetation are suitable for the restoration. We explore the influence of restored vegetation and re-emerged woodlands have on the process of soil rehabilitation and the increasing organic substance in it. We examine the impact of various combinations of vegetation on land rehabilitation. Explaining the degree of colonization of waste land by various types of vegetation helps us to reveal the trends of regenerative processes and identify the most promising for restoration tree species in affiliation with the ground vegetation. The results can assist decision-makers in choosing compatibility alliances of vegetation to sustain regeneration processes. Although this research is location specific, the knowledge developed can to a degree be applied to similar places, in the temperate zone.

Maria Kopiy, Albert Nijnik, Serhiy Kopiy, Leonid Kopiy, Maria Nijnik, Ruslana Presner, Igor Fizyk, Vasyl Agij, Ivanna Zholobchuk

01.12.2016.

Research paper

Seedling Quality in Serbia – Results from a Three-Year Survey

During a three-year survey in 14 forest nurseries in Serbia we measured seedlings of 19 species and 96 stocktypes of both conifers and broadleaves. Seedlings were measured in the nurseries for height and diameter, and subsamples was taken for measurements of shoot and root dry weight, and presence of mycorrhiza. Results of mean values and variation of measured morphological attributes are presented and compared to Serbian standard for seedlings quality. We found mycorrhiza on seedlings root from almost every nursery, seedbed and tray. Our results show that current nursery cultural practice need to be improved and that seedlings quality standards should be updated.

Vladan Ivetić, Zoran Maksimović, Ivona Kerkez, Jovana R Devetaković

01.12.2016.

Review paper

Direct Seeding in Reforestation – A Field Performance Review

Direct seeding has been considered a forest restoration option for centuries. Over the past half century, the use of this practice has declined in developed countries as forest regeneration programs have advanced with the production of quality seedlings that can successfully establish restoration sites. Direct seeding is being reconsidered as a restoration option as the potential size of the worldwide forest restoration program has grown because of massive deforestation in third-world nations and due to global climate change. This review examines direct seeding from a number of perspectives. First, merits of using this practice in restoration programs are defined. Major merits of this option are that it can be done quickly, over hard to reach and large disturbed areas, and at a relatively low cost. Second, current research findings from restoration programs are discussed. The major finding is that seedling establishment rates are low (i.e. typically around 20% of seeds planted) due to site conditions, seed predation and vegetation competition, and field performance (i.e. survival and growth) is lower than planted seedlings. Third, operational practices for the application in restoration programs are reviewed. To successfully conduct direct seeding programs practitioners need to consider seedbed receptivity, seed distribution and seeding rate. Fourth, potential new practices are presented. Some of these new practices attempt to create a more effective means to disperse seed across the site, minimize seed predation or create a more favorable microsite environment. This review provides a synthesis of what is known about direct seeding, thereby allowing practitioners to make a rational decision of whether to apply this practice towards their forest restoration program.

Steven C Grossnickle, Vladan Ivetić

01.12.2021.

Research paper

Sulfur and lime affect soil pH and nutrients in a sandy Pinus taeda nursery

Two pH experiments were conducted at a sandy, bareroot loblolly pine (Pinus taeda L.) nursery in Texas. A sulfur trial (0, 813, 1626, 2439 kg ha-1 of elemental sulfur) was installed to determine if lowering soil pH would result in nutrient toxicity symptoms and affect seedling morphology. Although soil acidity in the sulfur study ranged from pH 3.9 to pH 5.0, none of the treatments resulted in micronutrient toxicity and none affected height growth, root-collar diameter, root mass, shoot mass or the root-mass ratio (root dry mass/total dry mass). Acidifying soil with sulfur increased leaching of calcium, potassium, magnesium, manganese and zinc but there was no effect on seedling morphology. The objective of the liming trial (0, 813, 1626, 3252 kg ha-1 of dolomitic lime) was to determine if increasing alkalinity would result in an iron deficiency and reduce seedling growth. As expected, applying lime increased the calcium and magnesium levels but had no effect on soil levels of iron, phosphorus, potassium, sulfur, zinc and sodium. However, the root-mass ratio was reduced by applications of dolomitic lime (pH ranged from 5.3 to 6.0). Differences in soil properties (i.e. plot location) had a greater effect on seedling morphology than lime applications. Foliage levels of manganese and boron were reduced by the highest rate of lime and sulfur, respectively.

Ryan L Nadel, David B South, Scott A Enebak, Gene Bickerstaff

01.12.2016.

Review paper

Afforestation and reforestation in Romania: History, current practice and future perspectives

Preserving forest legacy was an important objective throughout Romanian history and so is today. Artificial forest regeneration (including both reforestation and afforestation) was widely used to ensure the forest continuity, following forest harvesting, or to create new forests on non-forest lands. As Romania has undergone various political changes that affected the socio-economic environment, these changes also left significant marks on forest cover dynamics. In this paper, we chronologically present the historical initiatives to preserve and increase forest cover in Romania, the current practices, available funding and future perspectives on artificial forest regeneration. Increasing forest cover from the current level of 27% to, and beyond European average of 33% seems an ambitious target for Romania. However, that may be achievable if the sources of funding for afforestation would diversify and increase, the available funds would be more effectively accessed, national forestry regulations would be less bureaucratic and the guidelines on artificial forest regeneration would be up to date and less restrictive. The whole framework of forestation should be revised to comply with recent social, economic and environmental realities, and modern criteria and indicators for sustainable forest management should be explicitly included. The new goals should also be tailored to the changes regarding the property type, climate related trends and new requirements of the forest certification systems.

Ciprian Palaghianu, Ioan Dutca

01.12.2016.

Research paper

Stocktype effect on field performance of Austrian pine seedlings

Austrian pine (Pinus nigra Arnold) seedlings are one of the most produced planting material in Serbian nurseries. In this study we compared a field performance of two container stocktypes, one usually used (2+0 produced in Plantagrah I) and one recently introduced (1+0 produced in Hiko V120 SS). The trial was established at planting site Vlaško polje (East Serbia), at altitude of 840 m a.s.l., one year following the total destruction of previous pine plantation by ice. One-year-old Austrian pine seedlings produced in Hiko containers show similar initial height and root collar diameter at planting time as two-year-old seedlings produced in traditionally used Plantagrah I containers. There was no vegetation control at field trial during the first growing season. At the end of the first growing season, taller (15.6 cm) and more slender (H/D=4.5) 1+0 seedlings from Hiko containers survived at higher rate, and shorter (10.9 cm) and more stocky seedlings 2+0 produced in Plantagrah I grow in height at higher rate. Seedlings taller at the planting time kept their advantage in size after the first growing season at the field. We found that both stocktypes can be used in operational planting programs on sites with lack of vegetation control.

Jovana R Devetaković, Zoran Makimović, Boris Ivanović, Zvonimir Baković, Vladan Ivetić

01.12.2016.

Research paper

Production of Acacia auriculiformis A. Cunn. ex Benth. for reforestation in southern Benin

The viability of Acacia auriculiformis production system in southern Benin was studied from an analytical perspective combining the production techniques and the financial profitability. The driving research question was as follows: "Will the nurseries be able to supply the seedlings suited to successful reforestation programmes?" A survey was carried out among nursery holders in the Atlantique Department. The snowball sampling method enabled to survey 55 nursery holders, based on semi-structured interviews. Respondents provided data on the organisation of production, production techniques, production costs, and sales revenues. There were two types of nurseries: individual nurseries and collective nurseries. The stages of acacia seedlings production were as follows: seed collection, dormancy breaking, sowing, and cares for young seedlings. Overall, the production techniques used by nursery holders were effective. The net revenue for 1000 seedlings ranged between XOF 9000 and 26000. Collective nurseries had lower production costs, hence higher revenues, compared to individual nurseries. It is essential to support nursery holders through capacity strengthening training and the availability of good quality seeds.

Augustin K. N. Aoudji, Caroline Séhouéto, Anselme Adégbidi, Rodrigue S. Kaki, Jean C. Ganglo

01.12.2016.

Research paper

Using double-sampling techniques to reduce the number of measurement trees during forest inventories

Variable-radius sampling techniques are commonly used during forest inventories. For each sample tree at a particular sampling point, diameter and height(s) are measured and then weight is estimated using established equations.  Heights can require a fair amount of time to measure in the field.  Separating the weight per acre estimate into two components; average basal area per acre and WBAR (individual tree weight-basal area ratio) across all points, can often lead to more efficient sampling schemes. Variable-radius sampling allows for a quick estimate of basal area per acre at a point since no individual tree measurements are needed.  If there is a strong relationship between weight and basal area, then by knowing basal area you essentially know weight.  Separation into two components is advantageous because in most cases there is more variability among basal area estimates per point then there is in WBAR. Hence, you can spend more resources establishing many points that only estimate basal area – often called “Count” points. “Full” points are those where individual tree measurements are also conducted. There is little published information quantifying the impacts on basal area, weight, etc., estimates among different “Full/Count” sample size ratios at the same site. Inventories were examined to determine this method’s applicability to loblolly pine plantations in southern Arkansas and northern Louisiana. Results show there is more variability among basal area estimates than WBAR and that the amount of trees being “intensively” measured is excessive.  Based on these four plantations, a “Full” point could be installed ranging from every other point to every fifth point depending on site conditions and the desired variable.

Curtis L. VanderSchaaf, Gordon Holley, Joshua Adams

01.12.2016.

Research paper

Should forest regeneration studies have more replications?

When it comes to testing for differences in seedling survival, researchers sometimes make a Type II statistical error (i.e. failure to reject a false null hypothesis) due to the inherent variability associated with survival in tree planting studies. For example, in one trial (with five replications) first-year survival of seedlings planted in October (42%) was not significantly different (alpha = 0.05) from those planted in December (69%). Did planting in a dry October truly have no effect on survival? Authors who make a Type II error might not be aware that as seedling survival decreases (down to an overall average of 50% survival), statistical power declines. As a result, the ability to declare an 8% difference as “significant” is very difficult when survival averages 90% or less.  We estimate that about half of regeneration trials (average survival of pines <90%) cannot declare a 12% difference as statistically significant (alpha = 0.05).  When researchers realize their tree planting trials have low statistical power, they should consider using more replications.  Other ways to increase power include: (1) use a one-tailed test (2) use a potentially more powerful contrast test (instead of an overall treatment F-test) and (3) conduct survival trials under a roof.

David B. South, Curtis L. VanderSchaaf

01.12.2016.

Review paper

Developing and supporting quality nursery facilities and staff are necessary to meet global forest and landscape restoration needs

Seedlings are the foundation for many terrestrial ecosystems and are a critical consideration and investment for implementing global forest and landscape restoration programs. Global leaders have pledged to restore millions of hectares during the next decade, necessitating many millions of established plants. Although natural regeneration and direct seeding will likely meet a portion of that need, large quantities of high-quality, nursery-grown seedlings are also required. Insufficient plant quantities or poor-quality plants result in unsuccessful outplanting programs. Such failures have considerable economic and environmental consequences and will result in an inability to meet restoration goals. Nonetheless, the importance of restoration nurseries is often overlooked when making large-scale restoration commitments. Technology already exists to produce high-quality plants to meet a variety of goals. This technology cannot be applied, however, unless adequate resources and training are made available by overcoming political and socioeconomic barriers. In this article, we discuss the important role of nurseries to meet global restoration commitments and review three case studies where increased support to nursery programs resulted in improved restoration success.

Diane L Haase, Anthony S Davis