The growth of one-year-old narrow-leaved ash seedlings is strongly related to the leaf area parameters

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Ivona Kerkez Jankovic
Mirjana Šijačić-Nikolić
Marina Nonić
Jovana Devetaković


The leaf area of plant leave scan be considered as an indicator of the plant growth conditions, and its variability is usually associated with adaptation and response to the changing environment. The aim of this study is to investigate the relation of the leaf area parameters on growth of the narrow-leaved ash 1+0 seedlings from the nursery trial and determining variability between the 18 half-sib lines. The seedlings were obtained from seeds collected from 18 maternal trees found in the area of the special nature reserve Upper Danube Region. Ten randomly selected 1+0 seedlings per half-sib line were selected for further study, and all leaves per seedling were collected, herbarized and scanned. The leaf area of each leaf (LACL) was measured using an open-source image processing program – ImageJ, and the total leaf area per seedling (TLA) was calculated. Also, the number of leaves per plant (NCL) as well as the number of leaflets within each imparipinnate compound leaf (NLCL) were counted. The results showed that differences between studied attributes were statistically significant among 18 selected half-sib lines (p < 0.05). The most variable parameter was the total leaf area per seedling (TLA; CV=68.98%), which is strongly correlated to the leaf area per each leaf (LACL; r=0.95) and the number of leaflets (NLCL; r=0.94). In this study, we confirmed that leaf parameters were highly correlated to the growth of one-year-old Fraxinus angustifolia seedlings.


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Kerkez Jankovic, Ivona, Mirjana Šijačić-Nikolić, Marina Nonić, and Jovana Devetaković. “The Growth of One-Year-Old Narrow-Leaved Ash Seedlings Is Strongly Related to the Leaf Area Parameters”. REFORESTA, no. 10 (December 30, 2020): 31-39. Accessed September 27, 2023.


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