Enhancement of seed germination rate and growth of Anagyris foetida L.
DOI:
https://doi.org/10.21750/REFOR.17.05.116Keywords:
Anagyris foetida, bioconservation, dormancy, Fabaceae, reforestation, seed coatAbstract
Bean trefoil (Anagyris foetida L.), the only species within the Anagyris genus (L.) of the Fabaceae family in Algeria, demonstrates considerable promise for reforestation actions in arid and semi-arid areas throughout the Mediterranean basin. Nevertheless, a prominent obstacle impeding the successful establishment of the species is the hardness of its seeds, resulting in reduced germination rates and inconsistent seedling emergence. The objective of this research is to improve seed germination and seedling development in A. foetida. A total of 750 pods were randomly collected from a population including 13 individuals. From this sample, 100 intact pods and 100 seeds were chosen for measurement and weight assessment. Three different scarification techniques were utilized as pretreatments: chemical scarification, involving varying durations of immersion in 98% concentrated sulphuric acid; thermal scarification, involving 24 hours of exposure to a water bath set at 35°C; and mechanical scarification, achieved through the use of abrasive paper. Each treatment comprised four replicates of 50 seeds, while untreated seeds were utilized as controls. Germination and initial seedling development were assessed after 30 days of cultivation. Statistical analysis revealed significant differences (p<0.001) among pretreatments concerning germination and seedling emergence. The highest germination percentages were observed following 120 minutes and 150 minutes of sulphuric acid soaking, with 86% and 91.5% of germination, respectively. The chemical pretreatments demonstrated correlations with shoot and root growth, as well as collar diameter. In contrast, the control group exhibited notably low germination (5.5%), while mechanical scarification resulted in a 55% germination rate. Seedling survival rates ranged from 93.7% to 99.4%. The seeds of A. foetida predominantly exhibit physical dormancy attributed to their hard and impermeable seed coat. These findings could be valuable for the generative reproduction of the species, especially for producing plants for afforestation/reforestation programs.
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References
Akbag HI (2021) Potential nutritive value of Anagyris foetida shrub for goats. Agroforestry Systems 95(1):191-200. https://doi.org/10.1007/s10457-020-00573-8
Alatürk F, Alpars T, Gökkus A, Coskun E, Akbag HI (2014) Seasonal changes in the nutrient contents of some shrub species. COMU Journal of Agriculture Faculty 2(1): 133-141.
Al-Banna L, Darwish R M, Aburjai T (2003) Effect of plant extracts and essential oils on root-knot nematodes. Phytopathologia Mediterranea 42: 123-128.
Ali HH, Tanveer A, Nadeem MA, Asghar HN (2011) Methods to break seed dormancy of Rhynchosia capitata, a summer annual weed. Chilean Journal of Agricultural Research 71(3): 483. https://doi.org/10.4067/s0718-58392011000300021
Avsar MD (2009) Effect of some pretreatments on seed germination of bean trefoil (Anagyris foetida L.), summer-deciduous shrub. Fresenius Environmental Bulletin 18(6): 1014-1017.
Avsar MD, Ok T (2010) Phenological observations on a bean trefoil (Anagyris foetida L.) population in the Kahramanmaras region, Turkey. Scientific Research and Essays 5(22): 3358-3362.
Baes PO, de Viana ML, Sühring S (2002) Germination in Prosopis ferox seeds: Effects of mechanical, chemical and biological scarificators. Journal of Arid Environments 50(1): 185-189. https://doi.org/10.1006/jare.2001.0859
Barton LV (1965) Dormancy in seeds imposed by the seed coat. In Differenzierung und Entwicklung/Differentiation and Development (pp. 2374-2392). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-50088-6_59
Baskin CC, Milberg P, Andersson L, Baskin JM (2000) Germination studies of three dwarf shrubs (Vaccinium, Ericaceae) of Northern Hemisphere coniferous forests. Canadian Journal of Botany 78(12): 1552-1560. https://doi.org/10.1139/b00-129
Bellakhdar J (1997) La pharmacopée marocaine traditionnelle : Médecine arabe ancienne et savoirs populaires. France: Ibis Press.
Bewley JD, Black M (1994) Seeds. In Seeds (pp. 1-33). Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1002-8_1
Bnouham M, Merhfour FZ, Elachoui M, Legssyer A, Mekhfi H, Lamnaouer D, Ziyyat A (2006) Toxic effects of some medicinal plants used in Moroccan traditional medicine. Moroccan Journal of Biology 2(3): 21-30.
Bonner FT (1984) New forests from better seeds: the role of seed physiology. In Seedling physiology and reforestation success (pp. 37-59). Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6137-1_3
Bradford KJ (2017) Water relations in seed germination. In Seed development and germination (pp. 351-396). Routledge. https://doi.org/10.1201/9780203740071-13
Cardinale M, Lanza A, Bonnì ML, Marsala S, Puglia AM, Quatrini P (2008) Diversity of rhizobia nodulating wild shrubs of Sicily and some neighbouring islands. Archives of Microbiology 190(4): 461-470. https://doi.org/10.1007/s00203-008-0394-2
Darwish RM, Aburjai TA (2010) Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli. BMC Complementary and Alternative Medicine 10(1):1-8. https://doi.org/10.1186/1472-6882-10-9
Eisvand HR, Arefi HM, Tavakol-Afshari R (2006) Effects of various treatments on breaking seed dormancy of Astragalus siliquosus. Seed Science and Technology 34(3): 747-752. https://doi.org/10.15258/sst.2006.34.3.22
Gonçalves EP, Soares FSDJ, Silva SDS, Tavares DDS, Viana JS, Cardoso BCC (2011) Dormancy breaking in Ormosia arborea Seeds. International Journal of Agronomy, Article ID 524709, 5 pp. https://doi.org/10.1155/2011/524709
Grossnickle SC, Ivetić V (2017) Direct seeding in reforestation–a field performance review. Reforesta 4: 94-142. https://doi.org/10.21750/refor.4.07.46
Houle G (1995) Seed dispersal and seedling recruitment: The missing link(s). Écoscience 2(3): 238-244. https://doi.org/10.1080/11956860.1995.11682289
Innocenti G, Dall'Acqua S, Viola G, Loi MC (2006) Cytotoxic constituents from Anagyris foetida leaves. Fitoterapia 77(7-8): 595-597. https://doi.org/10.1016/j.fitote.2006.06.012
ISTA (2005) International rules for seed testing. International Seed Testing Association. Bassersdorf, Switzerland. https://doi.org/10.15258/istarules.2015.i
Kamra SK (1990) Improving the forest seed situation in some African countries. Tropical Tree Seed Research 28:126-131.
Kheloufi A, Mansouri LM (2017) Effect of sulphuric acid on the germination of a forage tree Acacia nilotica (L.) subsp. tomentosa. Livestock Research for Rural Development 29(2).
Kheloufi A (2017) Germination of seeds from two leguminous trees (Acacia karroo and Gleditsia triacanthos) following different pre-treatments. Seed Science and Technology 45(1): 259-262. https://doi.org/10.15258/sst.2017.45.1.21
Kheloufi A (2019) Contribution à l’étude des effets de la sécheresse et du stress salin sur l’écophysiologie des espèces d’Acacia en Algérie. Thèse de Doctorat, Université de Batna 2., Batna, Algérie, 136p.
Kheloufi A, Boukhatem FZ, Mansouri LM, Djelilate M (2019) Maximizing seed germination in five species of the genus Acacia (Fabaceae Mimosaceae). Reforesta 7: 15-23. https://doi.org/10.21750/refor.7.02.64
Kheloufi A, Mansouri LM, Boukhatem FZ (2017) Application and use of sulphuric acid pretreatment to improve seed germination of three acacia species. Reforesta 3: 1-10. https://doi.org/10.21750/refor.3.01.25
Kheloufi A, Mansouri LM, Aziz N, Sahnoune M, Boukemiche S, Ababsa B (2018) Breaking seed coat dormancy of six tree species. Reforesta 5: 4-14. https://doi.org/10.21750/refor.5.02.48
Kheloufi A, Mansouri LM, Djelilate M, Touka M, Chater A, Dekhinet C (2020) Nutritional characteristics and seed germination improvement of the forage shrub Retama sphaerocarpa (L.) Boiss. Contemporary Agriculture 69(3-4): 53-60. https://doi.org/10.2478/contagri-2020-0008
Kildisheva OA, Dixon KW, Silveira FA, Chapman T, Di Sacco A, Mondoni A, Cross AT (2020) Dormancy and germination: making every seed count in restoration. Restoration Ecology 28: 256-265. https://doi.org/10.1111/rec.13140
Kimura E, Islam MA (2012) Seed scarification methods and their use in forage legumes. Research Journal of Seed Science 5(2): 38-50. https://doi.org/10.3923/rjss.2012.38.50
Maldonado-Arciniegas F, Ruales C, Caviedes M, Ramírez DX, León-Reyes A (2018) An evaluation of physical and mechanical scarification methods on seed germination of Vachellia macracantha (Humb. & Bonpl. ex Willd.) Seigler & Ebinger. Acta Agronómica 67(1): 120-125. https://doi.org/10.15446/acag.v67n1.60696
Mansouri L M, Kheloufi A, Belatreche R, Abdou I, Boukhatem ZF, Nouzha H (2020) Effects of nitrogen fertilization on mycorrhizal infection, nodulation and growth of Phaseolus vulgaris L. Contemporary Agriculture 69(3-4): 61-72. https://doi.org/10.2478/contagri-2020-0009
Nonogaki H (2019) Seed germination and dormancy: The classic story, new puzzles, and evolution. Journal of Integrative Plant Biology 61(5): 541-563. https://doi.org/10.1111/jipb.12762
Okunlola AI, Adebayo A, Orimogunje AD (2011) Methods of braking seed dormancy on germination and early seedling growth of African locust bean (Parkia Biglobosa) (Jacq.) Benth. Journal of Horticulture and Forestry 3(1): 1-6.
Pedrini S, Balestrazzi A, Madsen MD, Bhalsing K, Hardegree SP, Dixon KW, Kildisheva OA (2020) Seed enhancement: getting seeds restoration‐ready. Restoration Ecology 28: 266-275. https://doi.org/10.1111/rec.13184
Penfield S (2017) Seed dormancy and germination. Current Biology 27(17): 874-878. https://doi.org/10.1016/j.cub.2017.05.050
Rifna EJ, Ramanan KR, Mahendran R (2019) Emerging technology applications for improving seed germination. Trends in Food Science & Technology 86: 95-108. https://doi.org/10.1016/j.tifs.2019.02.029
Rusdy M (2017) A review on hard seedness and breaking dormancy in tropical forage legumes. Livestock Research for Rural Development 29: 12.
Tölü C, Yurtman IY, Baytekin H, Savas T (2012) Foraging strategies of goats in a pasture of wheat and scrubland. Animal Production Science 52(12): 1069-1076. https://doi.org/10.1071/an11251
Valtueña FJ, Ortega-Olivencia A, Rodriguez-Riano T (2007) Nectar production in Anagyris foetida (Fabaceae): two types of concentration in flowers with hanging droplet. International Journal of Plant Sciences 168(5): 627-638. https://doi.org/10.1086/513482
Valtueña FJ, Ortega-Olivencia A, Rodríguez-Riaño T (2008) Germination and seed bank biology in some Iberian populations of Anagyris foetida L. (Leguminosae). Plant Systematics and Evolution 275(3): 231-243. https://doi.org/10.1007/s00606-008-0067-2
Valtueña FJ, Ortega‐Olivencia A, Rodríguez‐Riaño T (2012) Regulation of fruit and seed set in Anagyris foetida L. (Fabaceae): The role of intrinsic factors. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology 146(sup1): 190-200. https://doi.org/10.1080/11263504.2012.679976
Wallace KJ, Clarkson BD (2019) Urban forest restoration ecology: a review from Hamilton, New Zealand. Journal of the Royal Society of New Zealand 49(3): 347-369. https://doi.org/10.1080/03036758.2019.1637352
Warlop F (2006) Limitation des populations de ravageurs de l'olivier par le recours à la lutte biologique par conservation. Cahiers Agricultures 15(5): 449-455.
WCD Tutiempo (2020) World Climate Data Tutiempo. Available at: https://fr.tutiempo.net/climat/2020/ws-604900.html (accessed 20.10.2020).
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